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Rad59 regulates association of Rad52 withDNAdouble‐strand breaks
| Content Provider | Scilit |
|---|---|
| Author | Pannunzio, Nicholas R. Manthey, Glenn M. Liddell, Lauren C. Fu, Becky Xu Hua Roberts, Cai M. Bailis, Adam M. |
| Copyright Year | 2012 |
| Description | Journal: MicrobiologyOpen Homologous recombination among repetitive sequences is an important mode of DNA repair in eukaryotes following acute radiation exposure. We have developed an assay in Saccharomyces cerevisiae that models how multiple DNA double-strand breaks form chromosomal translocations by a nonconservative homologous recombination mechanism, single-strand annealing, and identified the Rad52 paralog, Rad59, as an important factor. We show through genetic and molecular analyses that Rad59 possesses distinct Rad52-dependent and -independent functions, and that Rad59 plays a critical role in the localization of Rad52 to double-strand breaks. Our analysis further suggests that Rad52 and Rad59 act in multiple, sequential processes that determine genome structure following acute exposure to DNA damaging agents. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496973/pdf |
| Ending Page | 297 |
| Page Count | 13 |
| Starting Page | 285 |
| ISSN | 20458827 |
| e-ISSN | 20458827 |
| DOI | 10.1002/mbo3.31 |
| Journal | MicrobiologyOpen |
| Issue Number | 3 |
| Volume Number | 1 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2012-08-03 |
| Access Restriction | Open |
| Subject Keyword | Journal: MicrobiologyOpen Chromosomal Translocations Dna Double-strand Breaks Homologous Recombination Saccharomyces Cerevisiae Single-strand Annealing |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
