Loading...
Please wait, while we are loading the content...
Similar Documents
Centro De Pesquisa Aggeu Magalhães Mestrado Acadêmico Em Biociências E Biotecnologia Em Saúde
| Content Provider | Semantic Scholar |
|---|---|
| Copyright Year | 2014 |
| Abstract | This study aimed to characterize the molecular mechanisms of virulence and antimicrobial resistance in MDR K. pneumoniae isolates from a Teaching Hospital in Recife-PE. Six isolates of carbapenemase-producing K. pneumoniae were obtained from patients hospitalized in the ICU of a Teaching Hospital in Recife (Pernambuco, Brazil). Suscetibility testing was performed by CLSI broth microdilution. The isolates wereresistant to all β-lactams and quinolones tested. In contrast, susceptibility to amikacin, polymyxin B and tigecycline was detected. Molecular typing by PFGE revealed that the isolates were closely related, but did not belong to the same clone. Two differents STs were detected in the isolates, ST340 and ST11, both belonging to CC258. The blaKPC -2 and blaSHV-11 genes were detected in all isolates, followed by blaCTX-M 15 gene in four out of six isolates and, finally, blaCTX-M-2, qnrB19 , aac(6')-31 in two of six isolates . The blaKPC-2 and blaCTX-M-15 genes were present in the same 133 Kb plasmid belonging to IncI-γ in four isolates. In the remaining isolates blaKPC-2 and blaCTX-M-2 genes were also was contained in plasmid of nearly 133 Kb. However, was not possible to typecast this plasmid with the used methodology. The qnrB19 gene was detected being carried by 15 Kb plasmid belonging to IncY. All isolates presented class 1 integron, harboring aminoglycosides resistance genes. Mutations in the QRDR region of GyrA (Ser83Ile) and ParC (Ser80Ile) were detected in all isolates analyzed, this being the main mechanism of resistance to quinolones detected in this study. Additionally, membrane permeability was analyzed by SDSPAGE, verifying the absence of Ompk35 in all isolates. The OmpK36 was absence in only one isolate. The investigation of virulence revealed the presence of capsular antigens of type K1 between isolates. Genes encoding the fimbrial type I and III were detected, as well as genes involved in the synthesis of lipopolysaccharides and urease operon. The presence of multidrug resistant and virulent micro-organisms in hospitals, reinforces the need for measures for rapid containment of possibles nosocomial infections caused by these pathogens. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.arca.fiocruz.br/bitstream/icict/12169/1/22.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
