NDLI: In vitro plantlet regeneration from cotyledon, hypocotyl and root explants of hybrid seed geranium

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In vitro plantlet regeneration from cotyledon, hypocotyl and root explants of hybrid seed geranium

Content Provider	Semantic Scholar
Author	Chang, Charlene Moll, Ben A. Evenson, Kathleen B. Guiltinan, Mark J.
Copyright Year	2004
Abstract	In vitro plantlet regeneration systems for the seed geranium (Pelargonium x hortorum Bailey) using cotyledon, hypocotyl and root explants were optimized by studying the influence of seedling age, growth regulators and excision orientation on organogenesis. Indole-3-acetic acid combined with zeatin yielded the highest rate of shoot production on cotyledon explants (0.2–2 shoots per explant). More shoots were produced on explants cut from the most basal region of cotyledons from 2 to 4-day-old seedlings than from older seedlings or more distal cut sites. Hypocotyl explants produced the highest number of shoots, up to 40 shoots per explant, on indole-3-acetic acid (2.8–5.6 mM) + zeatin (4.6 mM) or thidiazuron (4.5 mM). Maximum shoot formation (0.3–1.4 shoots per explant) on root explants occurred when they were cultured on medium containing zeatin. Regenerated shoots rooted best on a basal medium containing no growth regulators. There were substantial differences among cultivars in shoot formation from each of the explant systems.
Starting Page	61
Ending Page	66
Page Count	6
File Format	PDF HTM / HTML
DOI	10.1007/BF00043429
Volume Number	45
Alternate Webpage(s)	http://www.personal.psu.edu/mjg9/Guiltinan_Lab_Website/Manuscripts_files/Chang%201996.pdf
Journal	Plant Cell, Tissue and Organ Culture
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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