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Regeneration of Platycodon grandiflorum Through Adventitious Shoots Formation From Cotyledon and Hypocotyl Explants
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yamamoto, Tomohide Hirao, Takeshi |
| Copyright Year | 2011 |
| Abstract | An efficient protocol for mass propagation of Platycodon grandiflorum ‘Samidare-murasaki’ and ‘Sentimental Blue’ was developed using explants divided into two halves of expanded cotyledon and hypocotyl segments. The direct formation of shoots from these explants was most active on the Murashige-Skoog (MS) medium supplemented with BA (1 mg∙L-1) and NAA (0.1 mg∙L-1). In this research, 20–23 shoots were initiated from cotyledon explant and 8–9 shoots were initiated from hypocotyl segment 6 weeks after the beginning of culture. Most of adventitious shoots were directly formed at the proximal cut end of proximal half of a cotyledon, and successively at the proximal cut end of distal half. The distal cut end of the hypocotyl was more active in the formation of adventitious shoots than the proximal cut end. Rooting of the adventitious shoots was promoted in the MS medium supplemented with IBA (1 mg∙L-1), while NAA (0.1 mg∙L-1) had little effect on the rooting. After acclimatization, the regenerated plants grew normally and flowered in a greenhouse. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://admin.ipps.org/uploads/57_162.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
