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Lectin-like oxidized low density lipoprotein receptor 1 (LOX-1) expression in human articular chondrocytes.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Simopoulou, Theodora Malizos, Konstantinos N. Tsezou, Aspasia |
| Copyright Year | 2007 |
| Abstract | OBJECTIVE To investigate the involvement of oxidized low density lipoprotein (ox-LDL) and the expression of its receptor lectin-like oxidized low density lipoprotein receptor 1 (LOX-1) in osteoarthritis, by determining the ox-LDL in synovial fluid and the expression of LOX-1 mRNA and protein in osteoarthritic as well as normal cartilage. In addition, the effect of ox-LDL on chondrocyte viability and the effect of ascorbic acid (a well-known anti-oxidant) on LOX-1 expression were studied. METHODS Fifteen patients were included in the study. Osteoarthritic articular cartilage was obtained from two distinct locations in the knee (n = 10) and hip (n = 5), specifically from weight-bearing and non-weight-bearing areas of the same joints. Five individuals were used as controls. mRNA and protein expression were studied by RT-PCR and immunofluorescence, respectively. Ox-LDL was measured in the synovial fluid and in paired serum samples from the patients using the ELISA method. RESULTS Ox-LDL was detected in the synovial fluid and its receptor LOX-1 was detected in cartilage from both weight-bearing and non-weight-bearing areas, whereas no LOX-1 expression was found in normal cartilage. Ox-LDL reduced chondrocyte viability in cell cultures, while the addition of ascorbic acid to osteoarthritic chondrocytes resulted in a decrease in LOX-1 mRNA expression. CONCLUSION The detection of LOX-1 mRNA and protein expression in osteoarthritic cartilage drawn from both weight-bearing and non-weight-bearing regions of the same patients suggest that LOX-1 may be involved in the progression and pathogenesis of osteoarthritis. |
| File Format | PDF HTM / HTML |
| PubMed reference number | 17888218 |
| Journal | Medline |
| Volume Number | 25 |
| Issue Number | 4 |
| Alternate Webpage(s) | http://www.clinexprheumatol.org/article.asp?a=3125 |
| Journal | Clinical and experimental rheumatology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
