Purification, crystallization and preliminary X-ray analysis of the ligand-binding domain of human lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1).

Content Provider	Europe PMC
Author	Ishigaki, Tomoko Ohki, Izuru Oyama, Takuji Machida, Sachiko Morikawa, Kousuke Tate, Shin-ichi
Copyright Year	2005
Description	Two different fragments of the ligand-binding domain of LOX-1, the major receptor for oxidized low-density lipoprotein (LDL) on endothelial cells, have been crystallized in different forms. One crystal form contains the disulfide-linked dimer, which is the form of the molecule present on the cell surface; the other contains a monomeric form of the receptor that lacks the cysteine residue necessary to form disulfide-linked homodimers. The crystal of the monomeric ligand-binding domain belongs to space group P212121, with unit-cell parameters a = 56.79, b = 67.57, c = 79.02 Å. The crystal of the dimeric form belongs to space group C2, with unit-cell parameters a = 70.86, b = 49.56, c = 76.73 Å, β = 98.59°. Data for the dimeric form of the LOX-1 ligand-binding domain have been collected to 2.4 Å. For the monomeric form of the ligand-binding domain, native, heavy-atom derivative and SeMet-derivative crystals have been obtained; their diffraction data have been measured to 3.0, 2.4 and 1.8 Å resolution, respectively. Two different fragments of the ligand-binding domain of LOX-1, the major receptor for oxidized low-density lipoprotein (LDL) on endothelial cells, have been crystallized in different forms.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC1952312&blobtype=pdf
Volume Number	61
PubMed Central reference number	PMC1952312
Issue Number	Pt 5
Issue Number	pt 5
PubMed reference number	16511086
Journal	Acta Crystallogr Sect F Struct Biol Cryst Commun
e-ISSN	17443091
DOI	10.1107/s1744309105012042
Language	English
Publisher	International Union of Crystallography
Publisher Date	2005-04-28
Access Restriction	Open
Rights License	© International Union of Crystallography 2005
Subject Keyword	lectin-like oxidized low-density lipoprotein receptor 1 ligand-binding domain
Content Type	Text
Resource Type	Article
Subject	Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology