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Sensitive ChIP-DSL technology reveals an extensive estrogen receptor alpha-binding program on human gene promoters.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kwon, Young-Soo Garcia-Bassets, Ivan Hutt, Kasey R. Cheng, Christine S. Jin, Mingjie Liu, Dongyan Benner, Chris Wang, Dong Ye, Zhen Bibikova, Marina Fan, Jian-Bing Duan, Lingxun Glass, Christopher K. Rosenfeld, Michael G. Fu, Xiang-Dong |
| Copyright Year | 2007 |
| Abstract | ChIP coupled with microarray provides a powerful tool to determine in vivo binding profiling of transcription factors to deduce regulatory circuitries in mammalian cells. Aiming at improving the specificity and sensitivity of such analysis, we developed a new technology called ChIP-DSL using the DNA selection and ligation (DSL) strategy, permitting robust analysis with much reduced materials compared with standard procedures. We profiled general and sequence-specific DNA binding transcription factors using a full human genome promoter array based on the ChIP-DSL technology, revealing an unprecedented number of the estrogen receptor (ERalpha) target genes in MCF-7 cells. Coupled with gene expression profiling, we found that only a fraction of these direct ERalpha target genes were highly responsive to estrogen and that the expression of those ERalpha-bound, estrogen-inducible genes was associated with breast cancer progression in humans. This study demonstrates the power of the ChIP-DSL technology in revealing regulatory gene expression programs that have been previously invisible in the human genome. |
| Starting Page | 2241 |
| Ending Page | 2243 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/104/12/4852.full.pdf?with-ds=yes |
| Alternate Webpage(s) | http://www.pnas.org/content/104/12/4852.full.pdf |
| PubMed reference number | 17360330v1 |
| Volume Number | 104 |
| Issue Number | 12 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Estrogen Receptor alpha Estrogens Gene Expression Profiling Ligation Mammals Mammary Neoplasms TRANSCRIPTION FACTOR dna binding promoter |
| Content Type | Text |
| Resource Type | Article |
