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DASH-2: flexible, low-cost, and high-throughput SNP genotyping by dynamic allele-specific hybridization on membrane arrays.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jobs, Magnus Howell, Walter Mathias Stromqvist, Linda Mayr, Torsten Brookes, Anthony J. |
| Copyright Year | 2003 |
| Abstract | Genotyping technologies need to be continually improved in terms of their flexibility, cost-efficiency, and throughput, to push forward genome variation analysis. To this end, we have leveraged the inherent simplicity of dynamic allele-specific hybridization (DASH) and coupled it to recent innovations of centrifugal arrays and iFRET. We have thereby created a new genotyping platform we term DASH-2, which we demonstrate and evaluate in this report. The system is highly flexible in many ways (any plate format, PCR multiplexing, serial and parallel array processing, spectral-multiplexing of hybridization probes), thus supporting a wide range of application scales and objectives. Precision is demonstrated to be in the range 99.8-100%, and assay costs are 0.05 USD or less per genotype assignment. DASH-2 thus provides a powerful new alternative for genotyping practice, which can be used without the need for expensive robotics support. |
| File Format | PDF HTM / HTML |
| DOI | 10.1101/gr.801103 |
| PubMed reference number | 12727908 |
| Journal | Medline |
| Volume Number | 13 |
| Issue Number | 5 |
| Alternate Webpage(s) | https://lra.le.ac.uk/bitstream/2381/2025/1/Genome%20Res.-2003-Jobs-916-24.pdf |
| Alternate Webpage(s) | https://doi.org/10.1101/gr.801103 |
| Journal | Genome research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
