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UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL FACULDADE DE VETERINÁRIA PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS VETERINÁRIAS Caracterização fenotípica e genotípica de amostras de Salmonella enterica sorovar Typhimurium isoladas de suínos no Rio Grande do Sul.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bessa, Marjô Cadó Alegre, Porto |
| Copyright Year | 2006 |
| Abstract | The identification of Salmonella contamination sources throughout the pork production chain can be achieved by genotypic and phenotypic characterization of isolates. Thus, the aim of this study was typing a set of 97 Salmonella enterica subsp. enterica ser. Typhimurium (ST) strains isolated from pigs slaughtered in three different slaughterhouses located in the State of Rio Grande do Sul, using phage typing, IS200 hybridization, antimicrobial susceptibility testing, spvR region amplification, repetitive sequence polymerase chain reaction (rep-PCR) and PFGE. Furthermore, ST strains were tested for the susceptibility to ammonium quaternary compounds and iodophor by the tube dilution test. ST strains were classified in 12 different phage types and DT177 was the most prevalent. A unique IS200 hybridization pattern predominated among the strains, and in three isolates the spvR region was detected. Most strains were resistant against tetracycline, sulfonamide and streptomycin. Resistance patterns were related to the slaughterhouse, where strains have been isolated. The rep-PCR, using REP and ERIC primers, demonstrated an unique pattern among the strains, while the PFGE method resulted in 12 different patterns. Sixty four strains presented a similar PFGE pattern. All strains were inhibited by the ammonium quaternary compound in the recommended dilution as well as in a higher dilution. Against the iodophor compound, used in the recommended dilution, four strains were resistant, while 59 were not inhibited when a higher dilution was tested. The association of phage typing and PFGE pattern resulted in a better strain discrimination, and was considered the most suitable for characterization of porcine Salmonella strains. Furthermore, clonal groups were identified among this set of strains, indicating possible common points in Salmonella transmission chain in southern Brazil. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.lume.ufrgs.br/bitstream/handle/10183/6269/000527822.pdf?isAllowed=y&sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
