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Induction of G 2 / M phase arrest and apoptosis by ZGDHU-1 in A 549 and RERF-LC-MA lung cancer cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shen, Xinfeng Zhen, Wu Chen, Su-Feng Chen, Yifei Ya-Ping, Lv Zhou, Yong-Lie |
| Copyright Year | 2016 |
| Abstract | Lung cancer is a major public health issue worldwide and is associated with high mortality and poor prognosis. Chemotherapy has the potential to reduce tumor size, increase operability and eradicate micrometastases; therefore, novel chemicals to treat lung cancer are urgently required. In the present study, the effects of N, N'-di-(m-methylphenyi)-3,6-dimethyl-1,4-dihydro-1,2,4, 5-tetrazine-1,4-dicarboamide (ZGDHu-1), a novel tetrazine derivative, were investigated in A549 and RERF-LC-MA lung cancer cells, and the underlying molecular mechanism of ZGDHu in treating lung cancer was determined. Following incubation with different concentrations of ZGDHu‐1, flow cytometry analysis results indicated that ZGDHu-1 could induce G2/mitotic (M) cell cycle arrest and apoptosis in A549 and RERF-LC-MA cells in a dose-dependent manner. Furthermore, western blot analysis demonstrated that the expression levels of G2/M regulatory molecules, including cyclin B1, Cdc2 and cell division cycle 25c, decreased following treatment with ZGDHu-1, whilst p53 expression increased. In addition, A549 and RERF-LC-MA cell apoptosis was induced by cell cycle arrest at the G2/M phase and through the downregulation of nuclear factor-κB. These results suggest that ZGDHu-1 may induce G2/M phase arrest and apoptosis of lung cancer cells, and may serve as a potential therapeutic drug for the treatment of lung |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.spandidos-publications.com/ol/12/2/989/download |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
