NDLI: Adenanthin induces G 2 / M arrest and apoptosis in human non-small-cell lung cancer cells

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Adenanthin induces G 2 / M arrest and apoptosis in human non-small-cell lung cancer cells

Content Provider	Semantic Scholar
Author	Hu, Anbang Li, Jin-Ping Fang, Yulin Li, Weiqing
Copyright Year	2019
Abstract	Background: Non-small cell lung cancer (NSCLC) is a frequently encountered primary malignancy that is often immune to conventional chemotherapy. New chemotherapeutic agents are vital in combating this increasingly problematic disease. A natural diterpenoid derivative extracted from Isodon adenanthus leaves, adenanthin, has been demonstrated to produce anti-leukemic activity by acting on peroxiredoxin I/II. However, its role in NCSLC has yet to be fully investigated. Methods: Colony formation assays and CCK-8 assays were employed to investigate the growth of NSCLC cells and healthy cells. Induction of cellular apoptosis or arrest of cell cycles were determined by flow cytometric analysis, based on the Hoechst 33342 staining method or annexin V/propidium iodide. Intracellular levels of proteins related to apoptosis and the cell cycle were quantified via Western blot assay. A pan-caspase inhibitor, Z-VAD-FMK, allowed blockage of caspase activity in NSCLC (A549 and H460) cells, prior to treatment with adenanthin. Nude mice xenografts were created by subcutaneous inoculation, allowing researchers to study the in vivo effects of adenanthin (20 mg/kg, 40 mg/kg) on H460 cells. Apoptosis from mice tissues was observed by TUNEL assay. Pathological histology of tumor tissues was detected by H & E staining. Results: NSCLC cells (A549 and H460) were particularly sensitive to the effects of adenanthin. Adenanthin also conferred minimal cytotoxic damage to healthy cells. Adenanthin blocked NSCLC cell proliferation by arresting cell cycle progression in the G2/M phase and caspase3-dependent apoptosis. Adenanthin was able to attenuate tumor growth in mouse xenograft models while being well-tolerated, having an overall effect of prolonging mice survival. Conclusion: Adenanthin may serve as a novel anti-cancer agent that is potent and selective in eliminating NSCLC tumor cells.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.ijcem.com/files/ijcem0083466.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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