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GTP and cytosol stimulate phosphoinositide hydrolysis in isolated platelet membranes.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Baldassare, Joseph J. Fisher, Gary J. |
| Copyright Year | 1986 |
| Abstract | Hydrolysis of polyphosphoinositides by phospholipase C was examined in isolated membranes prepared from [32P]labelled platelets. In the presence of GTP gamma S, thrombin increased the release of inositol triphosphate and inositol biphosphate approximately 500%. GTP gamma S alone stimulated release 2 fold. Maximal activation of thrombin-induced phosphoinositide hydrolysis was observed at 10 uM GTP. Although addition of calcium had no effect, 2 mM EGTA completely inhibited inositolphosphate release. Addition of high speed supernatant to [32P]labelled membranes stimulated the release of inositolphosphates. This hydrolysis was further enhanced by the addition of GTP. These data demonstrate that the breakdown of polyphosphoinositides in isolated platelet membranes is dependent on GTP and stimulated by platelet cytosol. |
| Starting Page | 195 |
| Ending Page | 204 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://deepblue.lib.umich.edu/bitstream/handle/2027.42/26137/0000213.pdf?isAllowed=y&sequence=1 |
| PubMed reference number | 3015130v1 |
| Volume Number | 137 |
| Issue Number | 2 |
| Journal | Biochemical and biophysical research communications |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 1-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase Blood Platelets Calcium Catabolism Cytoplasmic matrix Egtazic Acid Guanosine Triphosphate Interferon Type II Lindane Phosphatidylinositol Phosphates Phosphatidylinositols Phospholipase C Supernatant Tissue membrane inositol biphosphate inositol triphosphate |
| Content Type | Text |
| Resource Type | Article |
