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Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)☆
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mutiso, Joshua Muli Macharia, John Chege Taracha, Evans Gicheru, Michael Muita |
| Copyright Year | 2012 |
| Abstract | In a previous immunogenicity and efficacy study in mice, montanide ISA 720 (MISA) was indicated to be a better adjuvant than bacillus calmette guerin vaccine (BCG) for a Leishmania vaccine. In the present study, we report the safety, immunogenicity and efficacy of Leishmania donovani (L. donovani) sonicated antigen delivered with alum-BCG (AlBCG), MISA or monophosphoryl lipid A (MPLA) in vervet monkeys following intradermal inoculums. Vaccinated and control animals were challenged with virulent L. donovani parasites and the parasitic burden was determined. Only animals vaccinated with alum-BCG adversely reacted to the inoculum by producing ulcerative erythematous skin indurations. Non-parametric ANOVA followed by a post test showed significantly higher IgG antibodies, and revealed the presence of lymphoproliferative and interferon gamma responses in both AlBCG+Ag and MISA+Ag as compared to the MPLA+Ag or other groups (P < 0.001). We conclude that L. donovani sonicated antigen containing MISA is safe and is associated with protective immune response against Leishmania donovani infection in the vervet monkey model. |
| Starting Page | 8 |
| Ending Page | 16 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S1674-8301(12)60002-5 |
| PubMed reference number | 23554725 |
| Journal | Medline |
| Volume Number | 26 |
| Alternate Webpage(s) | http://ir-library.ku.ac.ke/bitstream/handle/123456789/11238/Leishmania%20donovani%20whole%20cell%20antigen%20delivered%20with%20adjuvants%20protects%20against%20visceral%20leishmaniasis%20in%20vervet%20monkeys%20(Chlorocebus%20aethiops).pdf?isAllowed=y&sequence=1 |
| Alternate Webpage(s) | https://ir-library.ku.ac.ke/bitstream/handle/123456789/11238/Leishmania%20donovani%20whole%20cell%20antigen%20delivered%20with%20adjuvants%20protects%20against%20visceral%20leishmaniasis.pdf?isAllowed=y&sequence=4 |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/da/89/jbr-26-01-008.PMC3596075.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/S1674-8301%2812%2960002-5 |
| Journal | Journal of biomedical research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
