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Vir typing: a long-PCR typing method for group A streptococci.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gardiner, Donald L. Hartas, Jon Currie, Bart J. Mathews, John D. Kemp, David J. Sriprakash, Kadaba Srinivasa |
| Copyright Year | 1995 |
| Abstract | We have developed a new procedure (Vir typing) for typing Streptococcus pyogenes, by amplifying the entire 5- to 7-kb variable vir regulon by long PCR. The amplified DNA is then cleaved with HaeIII and visualized by ethidium bromide fluorescence after agarose gel electrophoresis. A simple procedure for preparing DNA of sufficiently high quality from 96 samples was employed simultaneously. This DNA was also used to develop a random amplified polymorphic DNA (RAPD) procedure. The discriminatory power of the two DNA-based procedures was compared with previous methods, M typing, and multilocus enzyme electrophoresis. Both procedures were highly discriminatory, but the stoichiometric yield of restriction fragments in Vir typing allows unambiguous interpretation of results. |
| Starting Page | 188 |
| Ending Page | 192 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://genome.cshlp.org/content/4/5/288.full.pdf |
| PubMed reference number | 7580916v1 |
| Volume Number | 4 |
| Issue Number | 5 |
| Journal | PCR methods and applications |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Agarose Gel Cleaved cell Electrophoresis, Agar Gel Ethidium Bromide Fluorescence Gel Electrophoresis (lab technique) Preparation Random Amplified Polymorphic DNA Technique Regulon Streptococcus pyogenes |
| Content Type | Text |
| Resource Type | Article |
