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Characterization of divalent cation-induced [3H]Acetylcholine release from EGTA-treated rat hippocampal synaptosomes
| Content Provider | Semantic Scholar |
|---|---|
| Author | Vickroy, Thomas W. Schneider, C. J. |
| Copyright Year | 2004 |
| Abstract | Calcium-naive synaptosomes were used to assess the effects of divalent cations on [3H]acetylcholine release from rat hippocampal homogenates. Following equilibration with calcium-free buffer (containing 10μM EGTA), calcium reversibly increased [3H]acetylcholine efflux (up to five-fold) while causing no measurable efflux of lactate dehydrogenase. When substituted for calcium, strongtium and barium behaved similarly although barium exhibited three-fold greater efficacy. In the presence of elevated potassium, 4-aminopyridine or tetraethylammonium, the secretagogue efficacy of calcium (but not barium) was markedly increased. The release-promoting effects of both cations were inhibited by lanthanum, magnesium, cadmium, and ω-conotoxin but were insensitive to nifedipine and cobalt (both 10 μM). In addition, stimulation of muscarnic cholinergic autoreceptors substantially inhibited both calcium and barium-evoked [3H]acetylcholine release. Taken together, these results indicate that cation-evoked transmitter release from calcium-naive synaptosomes is subject to normal neuroregulatory mechanisms and therefore should be useful for investigating presynaptic modulation of neuronal exocytosis. |
| Starting Page | 1175 |
| Ending Page | 1185 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/BF00966598 |
| PubMed reference number | 1795760 |
| Journal | Medline |
| Volume Number | 16 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/BF00966598 |
| Alternate Webpage(s) | https://doi.org/10.1007/BF00966598 |
| Journal | Neurochemical Research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
