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Efecto de la inclusión de harina de langostilla (Pleuroncodes planipes) en el alimento sobre la expresión y actividad enzimática digestiva en el intestino del camarón blanco (Litopenaeus vannamei)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Borboa, Ayala Gladys, Elia |
| Copyright Year | 2014 |
| Abstract | Aquaculture is the sector with main consumption of fish meal, however this ingredient is expensive and is not always available, making it necessary to the exploration of new sources of protein are accessible and good quality. In Mexico, the Red Crab (Pleuroncodes planipes) is a natural resource with great potential to be used as a source of protein, allowing certain levels of inclusion in food accelerate growth, increase the proteolytic activity of the digestive gland and digestibility. The white shrimp is the main arable crustacean in Mexico, and still require studies to reduce problems related to diseases, learn more about their physiology and digestive Biochemistry at the molecular level. There have been many studies aimed to know digestive functions catabolic shrimp, based mainly on the digestive gland, however, little is known about the role of intestinal digestive enzymes. The objective of this study was to evaluate the effect of partial replacement of fish meal with red crab meal on activity and expression of the genes that code for the main digestive enzymes in the intestine of juvenile white shrimp, Litopenaeus vannamei. It conducted a bioassay of growth with shrimp (weight average 0.25 g) that were fed during 31 days with two foods: a food control containing fishmeal (HP) and a food containing red crab meal a way to replace the HP protein. At the end of the experiment, agencies in intermolt Stadium were weighed and slaughtered to dissect their intestines and perform analysis of gene expression by microarrays heterologous, above-expressed and repressed genes were obtained through a threshold set at 2 (Z score) in the software Genarise and analyzed in the server DAVID (Database for Annotation, Visualization and Integrated Discovery) to investigate the functionality of genes under and overexpressed in GO (Gene Ontology). The relative expression of selected genes was determined using qPCR using specific primers using the method ΔΔCq through the CFX96 system. The inclusion of red crab in feed produced higher growth and an increase in the proteolytic activity of trypsin and aminopeptidase type. The analysis of microarray generated revealed 416 overexpressed genes in the treatment with red crab meal, of which 227 are included in gene ontology. Our results show that the expression is higher in fed with red crab meal in trypsin and aminopeptidase shrimp. The patterns obtained from digestive proteases could result from a higher digestibility of protein and eventually affect the best growth obtained from shrimp fed with red crab meal. This work is the first study that shows the existence of genes coding for the major digestive enzymes in the intestine of shrimp L. vannamei. The results show that the Red crab can be considered as a source of high quality protein and a good partial substitute for fish meal or part of the ingredients of commercial diets, since it promotes the growth of shrimp through the better use of dietary nutrients and improves the digestive enzyme capacity. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://cibnor.repositorioinstitucional.mx/jspui/bitstream/1001/135/1/ayala_e.pdf |
| Alternate Webpage(s) | http://dspace.cibnor.mx:8080/bitstream/handle/123456789/430/ayala_e.pdf?isAllowed=y&sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
