Loading...
Please wait, while we are loading the content...
Similar Documents
CD4+-T-cell responses generated during murine Salmonella enterica serovar Typhimurium infection are directed towards multiple epitopes within the natural antigen FliC.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bergman, Molly Ann Cummings, Lisa A. Mayeda, Laura Fellnerova, Ivana |
| Copyright Year | 2005 |
| Abstract | The flagellar filament protein FliC is a natural antigen recognized by memory CD4+ T cells recovered from Salmonella enterica serovar Typhimurium-infected humans and mice. To further investigate T-cell responses to FliC, we derived FliC-specific CD4+-T-cell clones from mice of two different haplotypes following oral S. enterica serovar Typhimurium infection. Using C-terminal truncations of MalE-FliC recombinant fusion proteins, we mapped antigenic activity to four different regions of FliC; three of the four epitope-containing regions were present in both FliC and the alternate flagellin subunit FljB. We determined that two novel FliC epitopes were also present in flagellins from several gram-negative enteric bacterial species: E(k)-restricted FliC 80-94 (amino acids 80 to 94) and A(b)-restricted FliC 455-469. Further mapping confirmed the presence of two previously identified FliC epitopes: A(k)-restricted FliC 339-350 and A(b)-restricted FliC 428-442. Therefore, like the recognition site of the innate immune receptor Toll-like receptor 5, three of four FliC epitopes recognized by CD4+ T cells colocalize in the D0/D1 domains of FliC. Salmonella-infected macrophages and dendritic cells stimulated epitope-specific CD4+-T-cell proliferation; infected dendritic cells also activated T cells to produce gamma interferon. These data demonstrate that Salmonella infection generates murine CD4+-T-cell responses to multiple epitopes in the natural antigen FliC and that recognition of infected phagocytes by FliC-specific CD4+ T cells triggers effector functions known to be essential for protective immunity. Together, these data suggest that FliC-specific CD4+ T cells may contribute to cell-mediated host defenses against Salmonella. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://iai.asm.org/content/73/11/7226.full.pdf |
| PubMed reference number | 16239517v1 |
| Volume Number | 73 |
| Issue Number | 11 |
| Journal | Infection and immunity |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acids Antigens Cell Proliferation Cytoskeletal Filaments Eighty Epitopes Flagellin Haplotypes Interferon Type II Leukemia, B-Cell Phagocytes Precipitating Factors Receptors, Immunologic Recombinants Salmonella XXX Cult Salmonella enterica subsp. enterica serovar enteritidis Salmonella infections Serotype TLR4 protein, human Toll-Like Receptor 5 Toll-like receptors bacterial-type flagellum filament gram |
| Content Type | Text |
| Resource Type | Article |
