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An efficient method for ( Triticum aestivum ) the regeneration of wheat from anther cultures
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rr, P. Lreotv Serlleun, P. |
| Copyright Year | 2010 |
| Abstract | An efficient procedure to obtain high yield of green wheat plantlets from anther culture is presented. The technique was applied to 4 spring genotypes and 2 winter genotypes, using a modified potato-2 medium [CHueNc et al., l9'78), supplemented with glutamine, with sucrose being pretreated with activated charcoal, and agarose replacing agar. The total average percentage of anthers yielding at least one callus increased from 9.1 9o (with the original potato-2 medium) to 21 .6 7o vith our modified medium, while the total average percentage of regenerated green plants raised from 2.5 Yo (50 green plants from 2,040 anthers) to 6.9 Vo (369 green plants from 5,350 anthers). Sucrose pretreatment of excised anthers greatly increased the rate of callus induction for 2 winter genotypes (Fl hybrids). Introduction In some plant species, haploids are easily produced from anther cultures. However, for most gen€ra, including grasses, the rate of successful haploid production is low [Prcar.D et a\.,1978;Mrao et a\.,1978;Bl.rrreu,et ql,, l98l; Bulr-ocx et a\.,1982; FoRoucsr-WEHn et a\.,1982; GeNovESr and CoLLTNS, 1982; RrNes, 19831. (*) Laboratoire de Pathologie Végétale. Faculté des Sciences Agronomiques de I'Etat. Passage des Déportés, 2. 8-5800 Crvgloux (Belgique). (**) Chaire de Cénétique et d'Amélioration des Plantes. Faculté des Sciences Agronomiques de I'Etat. Passage des Déportés, 2. 8-5800 Grrrsloux (Belgique). (+*+) Centre de Lutte Intégrée en Phytopathologie. Faculté des Sciences Agronomiques de I'Etat. .Avenue Maréchal Juin, 13. 8-5800 GrNrsr-oux (Belgique). 302 ZHANG L.J., ANCEAU C., LEPOIVRE P., SEILLEUR P.. SEIV{AL J. In wheat, breeding of doubled-haploids is currently limited by the low frequency of plantlet production per anther, specially in eiire breeding rines [Burrocx et a|.,1982; PrcaRo and De BuysER, 1977], thus making it difficult to be used effectively by plant breeders [Gnrrrrxc, 1975]. Potato medium rvas much more effective in pollen callus induction than MS or Maiji I medium [Research Group 301, 1976], and has been widery used in many laboratories for anther cultures ol wheat, barley and rye [\\rxzel et a|.,1977; De Buvsen and HexRy, 1979,1980; Scri-TEFFER el at.,1979; SuNorRi-eND et al., 1979; Bur_locx et al., 19821. Cnu.c.Nc et al. 119781 designed a new porato medium named potaro-2 medium. The yield of pollen calli r'irh potaro-2 medium increased tivofold as compared to medium N6 [CHu, 19;-8] or Nlaiji l. porato-2 medium has been gradually adopted by many laborarories [OuveNc. 1986]. Our paper deals *'ith a modified medium, shotl.ing greater efficiencf in regenerating green plantlets than the original potaro-2 medium for 6 wheat genotypes. 2. Material and methods 2.1. N{ATERIAL Six genotypes ol field-groçn wheat {Triticam aesûvum) u'ere used as anther donors, i.e. spring genotypes Sabine, Line7, Atys and Echo, and *'inter genotypes Fl hybrid [(Zemon x V.P. Ml) Gema] x [(Zemon x Mer.50-B2t) Gema] HIl972, from the < Station d'Amélioration des Plantes, Gembloux Belgium > and N.R.P.B. 824997.H.1986.Fl ol NrcxeRsoN (\\'t and W2 respectively). 2.2. METHODS Donor plants were sown in mid-Nor-ember for *inter genotypes and lateApril for spring genotypes. No chemical treatmenr rvas applied in the test plots during the grorving period of donor plants. Preliminary tests were carried out ro shorv pos-iible variations betrveen pollen callus formation capacities of main spike or branched spikes of the same plant. In further experiments only the main spikes u-ere used as donors. we used anthers containing the midor lateuninucleate microspores, as described by Hr and ouv.q.xc tl981l. Anther stage \ïas defined according to that of the majority of the pollen. Young spikes were disinfected with 0.1q0 mercuric chloride for 8 min followed b-v 4 *'ashings in sterile distilled water [cHUANc et al., 1978]. After disinfection, intact anrhers were immediately removed from the spikes and placed in 9 cm diameter Petri dishes (about 120 anthers per dish) containing 30 ml ol solidified meCium. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://orbi.uliege.be/bitstream/2268/17347/1/N%C2%B0426%20-%20Lepoivre.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
