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Plant Regeneration from Embryogenic Calli, Cell Suspension Cultures and Protoplasts of Triticum aestivum L. (Wheat)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Vasil, Indra K. Vasil, Vimla Redway, Flona A. |
| Copyright Year | 1990 |
| Abstract | Efficient and reproducible methods were developed during the 1980's for plant regeneration from tissue cultures of all of the important species of the Gramineae (1,2). Regeneration of plants has been shown to be predominantly by the process of somatic embryogenesis (3). Embryogenic cultures can be initiated mostly from explants such as immature embryos, young inflorescences and bases of young leaves which contain meristematic and undifferentiated cells which are not yet committed to specific developmental pathways. Embryogenic cells are relatively small, isodiametric, richly cytoplasmic, strongly basophilic, and contain prominent nuclei, amyloplasts, lipid droplets and only very small vacuoles. Like meristematic cells, they maintain their genetic fidelity during culture and have a selective advantage over cytologically aberrant cells in forming somatic embryos and plants (4–7). Furthermore, it has been shown that somatic embryos develop — directly or indirectly — from single cells (8,9). Consequently, plants derived from embryogenic cultures are neither chimeras nor variants, but true clones. The single cell origin, non-chimeral nature and genetic fidelity of plants derived from somatic embryos are very attractive features for any genetic transformation system. |
| Starting Page | 33 |
| Ending Page | 37 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/978-94-009-2103-0_4 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/978-94-009-2103-0_4 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
