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The NMDA receptor antagonist MK-801 reduces capsaicin-induced c-fos expression within rat trigeminal nucleus caudalis
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mitsikostas, Dimos-Dimitrios D. Río, Margarita Sánchez Del Waeber, Christian Moskowitz, Michael Ann Cutrer, Fred Michael |
| Copyright Year | 1998 |
| Abstract | The effect of the N-methyl-D-aspartate (NMDA) receptor antagonist (5R, 10S)-(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclo-hepten-5,10-i mine hydrogen maleate (MK-801) was examined on c-fos-like immunoreactivity (c-fos-LI) in urethane-anesthetized Sprague-Dawley rats using a polyclonal antibody. C-fos, an indicator of neuronal activation, was assessed within the trigeminal nucleus caudalis (TNC), area postrema. lateral reticular and solitary tract nuclei 2 h after intracisternal injection of capsaicin. C-fos-positive cells were counted at three representative levels corresponding to obex, -2.05 mm and -6.45 mm in 18 tissue sections (50 microm). A weighted average was obtained reflecting total brainstem expression within lamina I, II of TNC using a recently validated method. Capsaicin (0.1, 1, 5, 10 and 15 nmol) caused a dose-dependent labeling of cells in lamina I, II at obex similar to that previously reported after intracisternal blood or carrageenin administration in rats and guinea pigs. MK-801 (0.3, 1 and 3 mg/kg) administered i.p. 30 min before capsaicin (5 nmol in 100 microl artificial CSF) reduced significantly and dose-dependently (12%, 36% and 47%, respectively) the c-fos-LI cells in TNC at each level from rostral to caudal but not in solitary tract, area postrema and lateral reticular nuclei, and for unexplained reasons, increased c-fos-LI within the inferior olive. These results suggest that NMDA receptors provide a potential therapeutic target for cephalic pain (e.g. migraine) due to trigeminovascular activation from meningeal afferents. |
| Starting Page | 239 |
| Ending Page | 248 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0304-3959(98)00051-7 |
| PubMed reference number | 9696479 |
| Journal | Medline |
| Volume Number | 76 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0304395998000517 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0304395998000517?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/S0304-3959%2898%2900051-7 |
| Journal | Pain |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
