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Single skeletal muscle fiber elastic and contractile characteristics in young and older men.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ochala, Julien Frontera, Walter R. Dorer, David J. Hoecke, Jacques Van Krivickas, Lisa S. |
| Copyright Year | 2007 |
| Abstract | The current investigation was designed to: (a) assess the impact of aging on elastic characteristics of single skeletal muscle fibers from young (N = 6) and older men (N = 6); and (b) correlate the potential changes, with the fiber contractile properties. Chemically skinned single muscle fibers (n = 235) from vastus lateralis muscle were maximally activated. Maximal force and cross-sectional area were measured, and specific force calculated. The slack test was used to measure maximal unloaded shortening velocity. A quick release of 0.15% of fiber length was applied to determine instantaneous stiffness. The myosin heavy chain isoform composition of each single fiber was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Aging induces changes in both fiber elasticity (i.e., increased instantaneous stiffness) and contractility (i.e., reduced specific force and unloaded shortening velocity) in type I and IIa fibers. However, the changes in fiber stiffness may not directly influence contractile characteristics alterations. |
| Starting Page | 2233 |
| Ending Page | 2237 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/biomedgerontology/62/4/10.1093_gerona_62.4.375/1/375.pdf?Expires=1492936404&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=XSLpKh8AekSUYLJ41OGymnqKnBj~lhLsukueZbpwpZeaeNTUAzIJKsPR4aRWJhrmf5yQfLb4w94-Yu1kz~ClangngcMMRyP43Ipho-GRXxjRVHtCkGcrVSSu7qfykHng5bVlxEBaSkMhOsFrajZM2xpWY1oBrQUO-yvF9PcqIW25uDHTak~a4pYp3IPK4X~ZIUOTGMahdxhb8S498qkXmUy2tHLRbWEKF9gs6s-kBP6ZOkel4RqSboWgtNyh5wY3AlDb7WKU9LiNl0NxzWksABQVStNYt-D-KeBESBd~7hM5dHuxasJzPH4U0aWNMyrzmtcRRkx8Mu2mxw9YVADIig__ |
| Alternate Webpage(s) | https://watermark.silverchair.com/375.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAlkwggJVBgkqhkiG9w0BBwagggJGMIICQgIBADCCAjsGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMe0sBd2tuJiMiOaA_AgEQgIICDLDn6UOD17hBiYfmdrreihYrE-5Z-WrqPyYT2Q8wfojn3IWeuCVNiaN5F1EiCvA_mNO1kpfAISEjJuLpzrjJ2PhSUPISj2M6N_DsoiH66f6Q3c1MsHv_WF2bUhVFR4viQ0aAZzD-66-aO1UleXfKkLRoqgqms8ozuKRWOGNjaRghlMSqOp2tfaq-QE5wj0xI_16IHy0huWivDV3NfhHyYZJbEBwlLWX_IMHgyIjVufTNw2EVPyvhTnKY2PSpXnxfNUfnbx17IMr8Yw88yn2URDwEUKHjp890cye2CYkoIidi87i9N-XNRhoE3NZBKp56Y5P7RWt-8PcHmiYuDUE0VzGPlraqxJ9D1Ugc1WOmPyHTcfzd8zaNyKWLg9SO0mXJY9grrxaVNIwMfQQ5w-rbGW0kHGYRwjrOzgrnGtZ5bPovkV8zCcc79oU1QtB4yNWCVDb0PVkYGiCVfeEsjyidgNI4Io4J3kyQEu66dki0KA2gtDJXCFpqFgCMylIbUYZYHuX4bJaBZp3I9A0IHgMkeydlwByG-UJTk-IbbJBm0jztRiiN7LqP6e2ynf4m0C9KXFUhaSRmqIaGEvO4NNDwX9bdma6Z25FaHrWmK7dxQjPY342eXxeFAJa_ns3SzO-nLgQiHqJRdCDA_RAt6lHDw1f0AxV1qz-3XppkFM20cgFTi8hPG7dkRVRlsfdF |
| Alternate Webpage(s) | http://citeseerx.ist.psu.edu/viewdoc/download;jsessionid=0721EBFD5FC384E06CA075FB5584D8C7?doi=10.1.1.491.4117&rep=rep1&type=pdf |
| PubMed reference number | 17452730v1 |
| Volume Number | 62 |
| Issue Number | 4 |
| Journal | The journals of gerontology. Series A, Biological sciences and medical sciences |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Actins Congenital Fiber Type Disproportion Exclusion Gel Electrophoresis (lab technique) Muscle Contraction Muscle Fibers Myosin ATPase Myosin Heavy Chains Polyacrylamide Gel Electrophoresis SDS-PAGE SLC3A2 gene Skeletal Myocytes Skin Smooth muscle (tissue) Sodium Dodecyl Sulfate Structure of vastus lateralis muscle Tendon structure Tissue fiber polyacrylamide gels |
| Content Type | Text |
| Resource Type | Article |
