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Myosin heavy chain profiles and body composition are different in old versus young Standardbred mares.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lehnhard, Robert A. McKeever, Kenneth Harrington Kearns, Charles F. Beekley, Matthew D. |
| Copyright Year | 2004 |
| Abstract | There are limited data on age-related changes in body composition or skeletal muscle in the horse. Therefore, the purpose of this study was to investigate any differences in muscle myosin heavy chain (MHC) and body composition associated with aging. Twenty-three young (4-8 years) and eight old (20+ years) unfit Standardbred mares were evaluated. Rump fat thickness was measured using B-mode ultrasound and per cent body fat (% fat) was calculated. Needle muscle biopsies were obtained from right gluteus medius muscle. MHC composition was determined via sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Three MHC isoforms were subsequently identified as type I, type IIA, and type IIX and quantified using a scanning and densometric system. There were no significant differences (p>0.05) between old and young mares in fat (%) (19.0+/-6.4 vs 20.5+/-5.4), fat mass (kg) (102.3+/-39.9 vs 106.9+/-37.1), or body weight (kg) (529.4+/-34.9 vs 512.7+/-57.7). However, the old mares had significantly (p<0.05) greater lean body mass than the young mares (427.1+/-24.5 vs 405.7+/-37.9). Aged mares had significantly (p<0.05) less type I (7.8+/-2.9% vs 12.1+/-4.4%) and IIA (27.8+/-7.1% vs 36.1+/-9.5%) fibres than the young group but more type IIX (64.6+/-4.7% vs 51.8+/-11.1%). The MHC data are consistent with the age-related changes seen in other species. |
| Starting Page | 59 |
| Ending Page | 66 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.equistat.co.uk/research/Ref10.pdf |
| Alternate Webpage(s) | http://apex.seraonline.org/APEXpdf/McKeever-AgeAndMuscle.pdf |
| PubMed reference number | 14623152v1 |
| Volume Number | 167 |
| Issue Number | 1 |
| Journal | Veterinary journal |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adipose tissue Aldosterone Biopsy Body Composition Endocrine System Diseases Exhibits as Topic Fat Measurement Gel Electrophoresis (lab technique) Growth Hormone Heart Atrium Hyperglycemia Hyperinsulinism Metabolic Process, Cellular Myalgia Myocytes, Smooth Muscle Myosin Heavy Chains Platelet Glycoprotein 4, human Polyacrylamide Gel Electrophoresis Protein Isoforms SLC3A2 gene Short hip rotator muscle Skeletal muscle structure Sodium Dodecyl Sulfate Structure of gluteus medius muscle Sulfate measurement Thyroid Diseases Thyroid Gland Follicular Adenoma Vasopressins blood pressure regulation lean body mass polyacrylamide gels |
| Content Type | Text |
| Resource Type | Article |
