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Analysis of the long-term actions of gabapentin and pregabalin in dorsal root ganglia and substantia gelatinosa.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Biggs, James E. Boakye, Paul Acheampong Ganesan, Naren Stemkowski, Patrick L. Lantero, Aquilino Ballanyi, Klaus Smith, Peter Anthony |
| Copyright Year | 2014 |
| Abstract | The α2δ-ligands pregabalin (PGB) and gabapentin (GBP) are used to treat neuropathic pain. We used whole cell recording to study their long-term effects on substantia gelatinosa and dorsal root ganglion (DRG) neurons. Spinal cord slices were prepared from embryonic day 13 rat embryos and maintained in organotypic culture for >5 wk (neuronal age equivalent to young adult rats). Exposure of similarly aged DRG neurons (dissociated and cultured from postnatal day 19 rats) to GBP or PGB for 5-6 days attenuated high-voltage-activated calcium channel currents (HVA ICa). Strong effects were seen in medium-sized and in small isolectin B4-negative (IB4-) DRG neurons, whereas large neurons and small neurons that bound isolectin B4 (IB4+) were hardly affected. GBP (100 μM) or PGB (10 μM) were less effective than 20 μM Mn(2+) in suppression of HVA ICa in small DRG neurons. By contrast, 5-6 days of exposure to these α2δ-ligands was more effective than 20 μM Mn(2+) in reducing spontaneous excitatory postsynaptic currents at synapses in substantia gelatinosa. Spinal actions of gabapentinoids cannot therefore be ascribed to decreased expression of HVA Ca(2+) channels in primary afferent nerve terminals. In substantia gelatinosa, 5-6 days of exposure to PGB was more effective in inhibiting excitatory synaptic drive to putative excitatory neurons than to putative inhibitory neurons. Although spontaneous inhibitory postsynaptic currents were also attenuated, the overall long-term effect of α2δ-ligands was to decrease network excitability as monitored by confocal Ca(2+) imaging. We suggest that selective actions of α2δ-ligands on populations of DRG neurons may predict their selective attenuation of excitatory transmission onto excitatory vs. inhibitory neurons in substantia gelatinosa. |
| Starting Page | 12 |
| Ending Page | 12 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jn.physiology.org/content/jn/112/10/2398.full.pdf |
| PubMed reference number | 25122705v1 |
| Alternate Webpage(s) | https://doi.org/10.1152/jn.00168.2014 |
| DOI | 10.1152/jn.00168.2014 |
| Journal | Journal of neurophysiology |
| Volume Number | 112 |
| Issue Number | 10 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Calcium Channel Coxsackievirus B4 Ab:ACnc:Pt:CSF:Qn Embryo Excitatory Postsynaptic Currents Ganglia Ganglia, Spinal Griffonia simplicifolia isolectin B4 Hippocampus (Brain) Homovanillic Acid Inhibition Inhibitory Postsynaptic Currents Isolectins Nerve Endings Neuralgia Neurons Posterior root of spinal nerve Postsynaptic Current Prostaglandins B Sensory nerve Substantia Gelatinosa Synapses Young Adult gabapentin pregabalin |
| Content Type | Text |
| Resource Type | Article |
