NDLI: Deregulated expression of A 1 , Bcl-2 , Bcl-xL , and Mcl-1 antiapoptotic proteins and Bid , Bad , and Bax proapoptotic genes in polycythemia vera patients

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Deregulated expression of A 1 , Bcl-2 , Bcl-xL , and Mcl-1 antiapoptotic proteins and Bid , Bad , and Bax proapoptotic genes in polycythemia vera patients

Content Provider	Semantic Scholar
Author	Gasparotto, Elainy Patrícia Lino Tognon, Raquel Ferreira, Aline F. Vilela, Gustavo Oliveira Palma, Patrícia Vianna Bonini Zanichelli, Maria Aparecida Souto, Elizabeth Xisto Velano, Carlos Eduardo Engel Simões, Belinda Pinto Carrara, Rita De Cássia Viu Simone Kashima Covas, Dimas Tadeu Castro, Fabíola Attié De Souza, Ana Maria Thiago De
Copyright Year	2012
Abstract	Apoptosis deregulation might have a role in the pathophysiology of polycythemia vera (PV). This study evaluated Bcl-2 molecule expression in CD34+ cells and leukocytes in 12 PV patients. Gene expression was investigated by real time PCR using SybrGreen Quantitect kit and protein expression was evaluated by western-blotting. JAK2 V617F mutation was detected according to Baxter et al (2005). CD34+ cells from PV patients presented higher levels of A1 and Mcl-1 expression (median: 22.6 and 5.2, respectively) in comparison with controls (0.9 and 0.5, p=0.004 and p=0.020); while Bcl-2 and Bcl-xL expression decreased in PV patients (0.18 and 1.19) compared with controls (1.39 and 2.01, p=0.006 and p=0.020). CD34+ cells in PV patients showed an elevated Bid expression (14.4) in comparison with healthy subjects (1.0; p=0.002). Patients’ leukocytes showed an A1 augmentation (7.41, p=0.001) and a reduced expression of Bax (0.19; p=0.040) and Bad (0.2; p=0.030). There was no correlation between JAK2 V617F allele burden and molecular expression. PV patients showed alterations in Bcl-2 members’ expression, which may interfere with control of apoptotic machinery and contribute to disease pathogenesis.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.scielo.br/pdf/bjps/v47n4/25.pdf
Language	English
Access Restriction	Open
Subject Keyword	Antiapoptotic Agent BAX gene BCL-XL Protein BCL2 gene Bad protein Baxter (robot) Computing the permanent Deregulation Gene Expression JAK2 NP_004963.1:p.V617F MCL1 gene Mast/Stem Cell Growth Factor Receptor Kit, human Mutation Patients Polycythemia Vera Real-Time Polymerase Chain Reaction protein expression
Content Type	Text
Resource Type	Article

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