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Heterogeneous antigenic properties of the porcine reproductive and respiratory syndrome virus nucleocapsid
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rappe, Julie Christiane Françoise García-Nicolás, Obdulio Flueckiger, Franziska Thuer, Barbara Hofmann, Martin Summerfield, Artur Ruggli, Nicolas |
| Copyright Year | 2016 |
| Abstract | Porcine reproductive and respiratory syndrome virus (PRRSV) is an arterivirus responsible for a widespread contagious disease of domestic pigs with high economic impact. Switzerland is one of the rare PRRSV-free countries in Europe, although sporadic outbreaks have occurred in the past. The PRRSV isolate IVI-1173 from the short outbreak in Switzerland in 2012 was entirely sequenced, and a functional full-length cDNA clone was constructed. Genetic and antigenic characterization of IVI-1173 revealed the importance of amino acid 90 of the nucleocapsid protein N as part of a conformational epitope. IVI-1173 was not detected by SDOW17, a monoclonal antibody against N widely used to detect PRRSV-infected cells. Substitution of alanine at position 90 of N [N(A90)] with a threonine [N(T90)] restored reactivity of vIVI1173-N(T90) to SDOW17 completely. The relevance of this amino acid for the conformational SDOW17 epitope of PRRSV N was further confirmed by the opposite substitution in a functional cDNA clone of the genotype 2 isolate RVB-581. Finally, N proteins from ten genotype 1 strains differing from threonine at position 90 were analysed for reactivity with SDOW17. N(A90) totally disrupted or severely affected the epitope in 7 out of 8 strains tested. Based on these findings, 225 genotype 1 strains were screened for the prevalence of N(A90). N(A90) is rare in classical subtype 1 and in subtype 3 strains, but is frequent in Russian subtype 1 (70%) and in subtype 2 (45%) isolates. In conclusion, this study highlights the variable antigenic properties of N among genotype 1 PRRSV strains. |
| Starting Page | 385 |
| Ending Page | 392 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://veterinaryresearch.biomedcentral.com/track/pdf/10.1186/s13567-016-0399-9?site=veterinaryresearch.biomedcentral.com |
| Alternate Webpage(s) | https://static-content.springer.com/esm/art:10.1186%2Fs13567-016-0399-9/MediaObjects/13567_2016_399_MOESM2_ESM.pdf |
| PubMed reference number | 27871316v1 |
| Volume Number | 47 |
| Journal | Veterinary research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Alanine Amino Acids Arterivirus Clone Communicable Diseases DNA, Complementary Measles Virus Nucleoprotein Monoclonal Antibodies Murine sarcoma viruses Porcine Reproductive and Respiratory Syndrome Porcine respiratory and reproductive syndrome virus Porcine species Reproduction Reproductive Techniques, Assisted Subtype (attribute) Sus scrofa domestica Threonine viral nucleocapsid location |
| Content Type | Text |
| Resource Type | Article |
