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Cadmium and calcium uptake in isolated mitochondria-rich cell populations from the gills of the freshwater rainbow trout.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gálvez, Fernando Espín Wong, Denise R. Wood, Chris M. |
| Copyright Year | 2006 |
| Abstract | A novel cell isolation technique was used to characterize cadmium and calcium uptake in distinct populations of gill cells from the adult rainbow trout (Oncorhynchus mykiss). A specific population of mitochondria-rich (MR) cell, termed the PNA+ MR cell (PNA is peanut lectin agglutinin), was found to accumulate over threefold more 109Cd than did PNA- MR cells, pavement cells (PV cells), and mucous cells during a 1-h in vivo exposure at 2.4 microg/l 109Cd. In vitro 109Cd exposures, performed in standard PBS and Cl- -free PBS, at concentrations from 1 to 16 microg/l 109Cd, were also carried out to further characterize Cd2+ uptake kinetics. As observed during in vivo experiments, PNA+ MR cells accumulated significantly more 109Cd than did other cell types when exposures were performed by an in vitro procedure in PBS. Under such conditions, Cd2+ accumulation kinetics in all cell types could be described with Michaelis-Menten relationships, with Km values of approximately 3.0 microg/l Cd (27 nM) for both MR cell subtypes and 8.6 microg/l Cd (77 nM) for PV cells. In similar experiments performed in Cl- -free conditions, a significant reduction in 109Cd accumulation in PNA+ MR cells was seen but not in PNA- MR or in PV cells. In vitro 45Ca fluxes were also performed to determine the cellular localization of Ca2+ transport in these functionally distinct populations of gill cells. 45Ca uptake was most pronounced in PNA+ MR cells, with levels over threefold higher than those found in either PNA(-) MR or in PV cells. Results from the present study suggest that the PNA+ MR cell type is a high-affinity and high-capacity site for apical entry of Cd2+ and Ca2+ in the gill epithelium of rainbow trout. |
| Starting Page | 19 |
| Ending Page | 26 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1152/ajpregu.00217.2005 |
| Alternate Webpage(s) | http://ajpregu.physiology.org/content/ajpregu/291/1/R170.full.pdf |
| Alternate Webpage(s) | http://www.lsu.edu/faculty/galvezf/Publications/galvezetal2006a.pdf |
| Alternate Webpage(s) | https://www.lsu.edu/faculty/galvezf/Publications/galvezetal2006a.pdf |
| Alternate Webpage(s) | https://www.zoology.ubc.ca/~woodcm/Woodblog/wp-content/uploads/2016/07/Galvez-et-al-2006-AJP.pdf |
| Alternate Webpage(s) | http://www.lsu.edu/faculty/galvezf/Publications/galvezetal2006.pdf |
| PubMed reference number | 16469841 |
| Alternate Webpage(s) | https://doi.org/10.1152/ajpregu.00217.2005 |
| Journal | Medline |
| Volume Number | 291 |
| Issue Number | 1 |
| Journal | American journal of physiology. Regulatory, integrative and comparative physiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
