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Isolation and characterization of mitochondria-rich cell types from the gill of freshwater rainbow trout.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gálvez, Fernando Espín Reid, Scott D. Hawkings, Guy Goss, Greg G. |
| Copyright Year | 2002 |
| Abstract | A magnetic cell separation technique (MACS) was developed for isolating and characterizing peanut lectin agglutinin positive (PNA(+)) cells from rainbow trout gills. Percoll density separated mitochondria-rich (MR) cells were serially labeled with PNA-FITC and an anti-FITC antibody covalently coupled to a 50-nm iron particle and then applied to a magnetic column. PNA(+) MR cells were enriched to >95% purity. Transmission electron microscopy analysis of both the PNA(+) and PNA negative (PNA(-)) fraction showed that PNA binds to MR chloride cells while the PNA(-) cell fraction is comprised of MR cells with features characteristic of pavement cells. Western blotting demonstrated that both PNA(+) and PNA(-) fractions had high levels of Na(+)-K(+)-ATPase and Sco1 expression; however, relative expression of H(+)-ATPase in PNA(+) and PNA(-) cells demonstrated that untreated fish had twofold higher H(+)-ATPase levels in PNA(-) cells relative to the PNA(+) cells. Furthermore, hypercapnic acidosis significantly increased the relative H(+)-ATPase expression on PNA(-) cells only, whereas metabolic alkalosis had no significant effect. |
| Starting Page | 1 |
| Ending Page | 14 |
| Page Count | 14 |
| File Format | PDF HTM / HTML |
| DOI | 10.1152/ajpregu.00342.2001 |
| PubMed reference number | 11832384 |
| Journal | Medline |
| Volume Number | 282 |
| Issue Number | 3 |
| Alternate Webpage(s) | http://ajpregu.physiology.org/content/ajpregu/282/3/R658.full.pdf |
| Alternate Webpage(s) | https://sites01.lsu.edu/faculty/galvezf/wp-content/uploads/sites/15/2015/06/Galvez-et-al.-2001.pdf |
| Alternate Webpage(s) | https://doi.org/10.1152/ajpregu.00342.2001 |
| Journal | American journal of physiology. Regulatory, integrative and comparative physiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
