NDLI: Ypk1 and Ypk2 kinases maintain Rho1 at the plasma membrane by flippase-dependent lipid remodeling after membrane stresses.

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Ypk1 and Ypk2 kinases maintain Rho1 at the plasma membrane by flippase-dependent lipid remodeling after membrane stresses.

Content Provider	Semantic Scholar
Author	Hatakeyama, Riko Kono, Keiko Yoshida, Satoshi Ohta-ku
Copyright Year	2017
Abstract	The plasma membrane (PM) is frequently challenged by mechanical stresses. In budding yeast, TORC2-Ypk1/Ypk2 kinase cascade plays a crucial role in PM stress responses by reorganizing the actin cytoskeleton via Rho1 GTPase. However, the molecular mechanism by which TORC2-Ypk1/Ypk2 regulates Rho1 is not well defined. Here, we found that Ypk1/Ypk2 maintain PM localization of Rho1 under PM stress via spatial reorganization of the lipids including phosphatidylserine. Genetic evidence suggests that this process is mediated by the Lem3-containing lipid flippase. We propose that lipid remodeling mediated by the TORC2-Ypk1/Ypk2-Lem3 axis is a backup mechanism for PM anchoring of Rho1 after PM stress-induced acute degradation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], which is responsible for Rho1 localization under normal conditions. Since all the signaling molecules studied here are conserved in higher eukaryotes, our findings might represent a general mechanism to cope with PM stress.
Starting Page	1169
Ending Page	1178
Page Count	10
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://jcs.biologists.org/content/joces/130/6/1169.full.pdf
PubMed reference number	28167678v1
Alternate Webpage(s)	https://doi.org/10.1242/jcs.198382
DOI	10.1242/jcs.198382
Journal	Journal of cell science
Volume Number	130
Issue Number	6
Language	English
Access Restriction	Open
Subject Keyword	Cascade Device Component Genus Axis Microfilaments Phosphatidylinositol 4,5-Diphosphate Phosphatidylserines Plasma membrane Stress, Mechanical Tissue membrane flippase
Content Type	Text
Resource Type	Article

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