NDLI: PtdIns(4,5)P2 turnover is required for multiple stages during clathrin- and actin-dependent endocytic internalization

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PtdIns(4,5)P2 turnover is required for multiple stages during clathrin- and actin-dependent endocytic internalization

Content Provider	Semantic Scholar
Author	Sun, Yidi Kaksonen, Marko Drubin, David G.
Copyright Year	2007
Abstract	The lipid phosphatidylinositol-4,5-bisphosphate (PtdIns[4,5]P(2)) appears to play an important role in endocytosis. However, the timing of its formation and turnover, and its specific functions at different stages during endocytic internalization, have not been established. In this study, Sla2 ANTH-GFP and Sjl2-3GFP were expressed as functional fusion proteins at endogenous levels to quantitatively explore PtdIns(4,5)P(2) dynamics during endocytosis in yeast. Our results indicate that PtdIns(4,5)P(2) levels increase and decline in conjunction with coat and actin assembly and disassembly, respectively. Live-cell image analysis of endocytic protein dynamics in an sjl1Delta sjl2Delta mutant, which has elevated PtdIns(4,5)P(2) levels, revealed that the endocytic machinery is still able to assemble and disassemble dynamically, albeit nonproductively. The defects in the dynamic behavior of the various endocytic proteins in this double mutant suggest that PtdIns(4,5)P(2) turnover is required for multiple stages during endocytic vesicle formation. Furthermore, our results indicate that PtdIns(4,5)P(2) turnover may act in coordination with the Ark1/Prk1 protein kinases in stimulating disassembly of the endocytic machinery.
Starting Page	355
Ending Page	367
Page Count	13
File Format	PDF HTM / HTML
PubMed reference number	17452534
Volume Number	177
Journal	The Journal of cell biology
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/177/2/355.full.pdf?with-ds=yes
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/02/f8/jcb1770355.PMC2064142.pdf
Alternate Webpage(s)	https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/02/f8/jcb1770355.PMC2064142.pdf
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/177/2/355.full.pdf
Language	English
Access Restriction	Open
Subject Keyword	Disassembly (action) Early endosome Green Fluorescent Proteins Phosphatidylinositol 4,5-Diphosphate Protein Kinases Stimulation (motivation) membrane budding
Content Type	Text
Resource Type	Article

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