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Solid-Phase Peptide Synthesis of Arginine-vasopressin with Amide Side-chain of Asparagine Protected with 1-Tetralinyl Group
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yusuf, A. O. Gitu, Peter M. Bhatt, B. Njogu, Martin Kagiki Salim, Ali Orata, Duke |
| Copyright Year | 2014 |
| Abstract | Arginine-vasopressin, a nonapeptide amide, was synthesized on a benzhydryl-resin using the Boc-strategy. Benzyl group was used in the protection of sulfhydryl group of cysteine and tyrosine side-chain. Benzhydryl, tetralinyl and tosyl groups were used in the protection of glutamine, asparagine and arginine side-chains respectively. TFMSA-TFA-thioanisole-1,2-ethanedithiol (2:20:2:1 v/v) was used to cleave the peptide from the resin under different conditions to obtain arginine-vasopressin in a one-pot reaction. The cleavage at 40°C for two hours gave arginine-vasopressin quantitatively (77% yield) Keywords : Solid-Phase Peptide Synthesis, resin, protecting group, cleavage, nonapeptide |
| Starting Page | 60 |
| Ending Page | 65 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Volume Number | 6 |
| Alternate Webpage(s) | https://profiles.uonbi.ac.ke/dorata/files/scan0010.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |