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Rhizobium leguminosarum has a second general amino acid permease with unusually broad substrate specificity and high similarity to branched-chain amino acid transporters (Bra/LIV) of the ABC family.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hosie, Arthur H. F. Allaway, David Galloway, Caroline S. Dunsby, H. A. Poole, Philip S. |
| Copyright Year | 2002 |
| Abstract | Amino acid uptake by Rhizobium leguminosarum is dominated by two ABC transporters, the general amino acid permease (Aap) and the branched-chain amino acid permease (Bra(Rl)). Characterization of the solute specificity of Bra(Rl) shows it to be the second general amino acid permease of R. leguminosarum. Although Bra(Rl) has high sequence identity to members of the family of hydrophobic amino acid transporters (HAAT), it transports a broad range of solutes, including acidic and basic polar amino acids (L-glutamate, L-arginine, and L-histidine), in addition to neutral amino acids (L-alanine and L-leucine). While amino and carboxyl groups are required for transport, solutes do not have to be alpha-amino acids. Consistent with this, Bra(Rl) is the first ABC transporter to be shown to transport gamma-aminobutyric acid (GABA). All previously identified bacterial GABA transporters are secondary carriers of the amino acid-polyamine-organocation (APC) superfamily. Also, transport by Bra(Rl) does not appear to be stereospecific as D amino acids cause significant inhibition of uptake of L-glutamate and L-leucine. Unlike all other solutes tested, L-alanine uptake is not dependent on solute binding protein BraC(Rl). Therefore, a second, unidentified solute binding protein may interact with the BraDEFG(Rl) membrane complex during L-alanine uptake. Overall, the data indicate that Bra(Rl) is a general amino acid permease of the HAAT family. Furthermore, Bra(Rl) has the broadest solute specificity of any characterized bacterial amino acid transporter. |
| Starting Page | 776 |
| Ending Page | 779 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.reading.ac.uk/AcaDepts/sb/rhizobium/psp/bra_paper_jbact_2002.pdf |
| PubMed reference number | 12107123v1 |
| Volume Number | 184 |
| Issue Number | 15 |
| Journal | Journal of bacteriology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Alanine Transaminase Amino Acid Permease Amino Acid Transporter Amino Acids Amino Acids, Branched-Chain Amino Acids, Neutral Arginine Carboxyl Group D-Alanine Transaminase Folic Acid Transporters GABA transporter Glutamic Acid Histidine Ammonia-Lyase Leucine Membrane Transport Proteins Polyamines Substrate Specificity Thioctic Acid alpha-Mannosidosis gamma-Aminobutyric Acid solute |
| Content Type | Text |
| Resource Type | Article |
