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The localization of chitin synthase in membranous vesicles (chitosomes) in Neurospora crassa.

Content Provider	Semantic Scholar
Author	Sietsma, Johannes H. Din, A. Beth Ziv, Vivi Sjollema, Klaas A. Yarden, Oded
Copyright Year	1996
Abstract	Polyclonal anti-chitin synthase antibodies raised against the Saccharomyces cerevisiae CHS2 gene product were used to identify and localize chitin synthase in the filamentous ascomycete Neurospora crassa. A single band of approximately 110 kDa was observed in Western blots of total protein extracts of N. crassa, probed with these antibodies. However, several additional bands were labelled when membrane fraction proteins (microsomes) were probed. Histo-immunochemical localization of chitin synthase confirmed that the polypeptide is compartmentalized in membranous vesicles (chitosomes), which are abundant in the vicinity of the hyphal tip. TEM analysis did not reveal chitin synthase in the plasma membrane. However, dense labelling of membrane-associated chitin synthase was observed by light-microscopic analysis of N. crassa protoplasts and at young hyphal tips.
Starting Page	202
Ending Page	206
Page Count	5
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://mic.sgmjournals.org/content/142/7/1591.full.pdf
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/micro/142/7/mic-142-7-1591.pdf?accname=guest&checksum=51A5F66531FFD4718D64E084CDAB0189&expires=1542778786&id=id
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/micro/142/7/mic-142-7-1591.pdf?accname=guest&checksum=19BBB6BCC9D323461650D096644A2157&expires=1545185455&id=id
PubMed reference number	8757723v1
Volume Number	142
Part	7
Journal	Microbiology
Language	English
Access Restriction	Open
Subject Keyword	Ascomycota (fungus) Bands Chitin Synthase Genome Encoded Entity Microsomes Neurospora sitophila Ab.IgE:ACnc:Pt:Ser:Qn Plasma membrane Polypeptides Protoplasts Tissue membrane Vesicle (morphologic abnormality) hyphal tip
Content Type	Text
Resource Type	Article

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