NDLI: Unreplicated DNA in mitosis precludes condensin binding and chromosome condensation in S. cerevisiae.

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Unreplicated DNA in mitosis precludes condensin binding and chromosome condensation in S. cerevisiae.

Content Provider	Semantic Scholar
Author	Dulev, Stanimir Aragón, Luís Strunnikov, Alexander
Copyright Year	2008
Abstract	Condensin is the core activity responsible for chromosome condensation in mitosis. In the yeast S. cerevisiae, condensin binding is enriched at the regions where DNA replication terminates. Therefore, we investigated whether DNA replication completion determines the condensin-binding proficiency of chromatin. In order to fulfill putative mitotic requirements for condensin activity we analyzed chromosome condensation and condensin binding to unreplicated chromosomes in mitosis. For this purpose we used pGAL:CDC6 cdc15-ts cells that are known to enter mitosis without DNA replication if CDC6 transcription is repressed prior to S-phase. Both the condensation of nucleolar chromatin and proper condensin targeting to rDNA sites failed when unreplicated chromosomes were driven in mitosis. We propose that the DNA replication results in structural and/or biochemical changes to replicated chromatin, which are required for two-phase condensin binding and proper chromosome condensation.
Starting Page	482
Ending Page	485
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.bioscience.org/fbs/getfile.php?FileName=/2008/v13/af/3120/3120.pdf
PubMed reference number	18508626v1
Volume Number	13
Journal	Frontiers in bioscience : a journal and virtual library
Language	English
Access Restriction	Open
Subject Keyword	CDC6 protein, human Chromosome Condensation Chromosomes DNA Replication Mitosis Saccharomyces cerevisiae Ab.IgA:ACnc:Pt:Ser:Qn condensin complexes nucleolar chromatin poly(glutamic acid-lysine)
Content Type	Text
Resource Type	Article

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