Loading...
Please wait, while we are loading the content...
Similar Documents
Interaction of anion exchanger 1 and glycophorin A in human erythroleukaemic K562 cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Pang, Allison J. Reithmeier, Reinhart A. F. |
| Copyright Year | 2009 |
| Abstract | AE1 [anion exchanger 1, also known as SLC4A1 (solute carrier family 4, anion exchanger, member 1) and band 3 (erythrocyte membrane protein band 3)] is a major membrane glycoprotein expressed in human erythrocytes where it mediates the exchange of chloride and bicarbonate across the plasma membrane. Glycophorin A (GPA) is a sialoglycoprotein that associates with AE1 in erythrocytes forming the Wrb (Wright b) blood group antigen. These two integral proteins may also form a complex during biosynthesis, with GPA facilitating the cell surface expression of AE1. This study investigates the interaction of GPA with AE1 in K562 cells, a human erythroleukaemic cell line that expresses GPA, and the role of GPA in the cell surface expression of AE1. In K562 cells, GPA was dimeric and N- and O-glycosylated similar to erythroid GPA. GPA was localized at the cell surface, but also localized to the Golgi. AE1 expressed in K562 cells contained both complex and high-mannose oligosaccharides, and co-localized with GPA at the cell surface and in the endoplasmic reticulum (ER). The Wrb antigen was detected at the cell surface of AE1-transfected K562 cells, indicating the existence of an AE1-GPA complex. Immunofluorescence and co-immunoprecipitation studies using AE1 and an ER-localized hereditary spherocytosis mutant (R760Q AE1) showed that GPA and AE1 could interact in the ER. GPA knockdown by shRNAs (small-hairpin RNAs), however, had no effect on the level of cell surface expression of AE1. The results indicate that AE1 and GPA form a complex in the ER of human K562 cells, but that both proteins can also traffic to the cell surface independently of each other. |
| Starting Page | 1 |
| Ending Page | 9 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.biochemj.org/content/ppbiochemj/421/3/345.full.pdf |
| PubMed reference number | 19438409v1 |
| Alternate Webpage(s) | https://doi.org/10.1042/BJ20090345 |
| DOI | 10.1042/bj20090345 |
| Journal | The Biochemical journal |
| Volume Number | 421 |
| Issue Number | 3 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Anabolism Anion Exchange Protein 1, Erythrocyte Anions Contain (action) Endoplasmic Reticulum Erythrocyte Membrane Erythrocytes Glycophorin A Hereditary spherocytosis K-562 Membrane Glycoproteins Membrane Proteins Oligosaccharides Plasma membrane SLC4A1 gene SLC4A1 wt Allele Sialoglycoproteins Solute Carrier Family Solute Carrier Organic Anion Transporter Family Member 1b1 |
| Content Type | Text |
| Resource Type | Article |
