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Engagement of CD45 during in vitro priming enhances antigen-specific Th cell frequencies.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Harris, Paul Emerson Liu, Zhuoru Colovai, Adriana Ioana Kinne, J. Maffei, Antonella Febles, A. Suciu-Foca, Nicole |
| Copyright Year | 1996 |
| Abstract | CD45 is a transmembrane protein tyrosine phosphatase expressed by all lymphoid cells including T cells. Substantial experimental data has shown that CD45 maintains a permissive state for TCR signaling. The highly glycosylated extracellular domain of CD45 may be the site of interaction with regulatory lectin-like counter-receptors on antigen-presenting cells. The mAb NDA5, recognizing a unique but broadly distributed epitope of CD45, was used to study the possible immunoregulatory role of CD45 during anti-CD3 and antigen-specific CD4+ T cell activation. In vitro priming of peripheral blood mononuclear cells with peptide antigens in the presence of mAb NDA5 results in a higher frequency of antigen-specific T cells. The responses of both naive and memory T cells to peptide antigens were sensitive to mAb NDA5-enhanced priming. Anti-CD3 activation of normal resting T cells, in the presence of mAb NDA5, resulted in enhancement of tyrosine phosphorylation of specific intracellular proteins associated with TCR signal transduction. In cultures without antigen, mAb NDA5 down-regulated the cell surface expression of both CD3 and CD4, yet did not stimulate proliferation of resting T cells. Together these results suggest that engagement of CD45 during in vitro priming has a significant effect on the development of antigen-specific T cell populations. |
| Starting Page | 85 |
| Ending Page | 88 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/intimm/8/8/10.1093/intimm/8.8.1265/2/8-8-1265.pdf?Expires=1491286875&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=aV2gZCKdkSRoQTVjt97Ap69Ft-rqCI1UJbmckv8lDcH~eEhwIx5Mwe~swc2AVep7BRdfss4-NDao0AOXLcqv6hCICOSDnFOCflExGXtqIB8nAR1e-xGk8QMlLxqjh-dpJA~RYySrjdFggZJDJYe6xBP57qyMT2H8JPOVVGYSQ2IyCJ~oZ9F~kiIakJDSCzNMOV8-~ZbEueITE1K7EQ~rfWbKZxPh2uIFjm0gHco7JjCeOiwJyFaFSq-CBFUMKH-p7KTLvM0sDlIrWN0tmuhvB85uO66kvJ7yOgpwoSwQii27arirnrIGWDeWIJ6plKTWLHvlqkDjWrr18u7HFyi2PQ__ |
| PubMed reference number | 8918696v1 |
| Volume Number | 8 |
| Issue Number | 8 |
| Journal | International immunology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | CD45 Antigens Cellular Phone DC101 monoclonal antibody Helper-Inducer T-Lymphocyte In Vitro [Publication Type] Integral Membrane Proteins Interphase Cell Lectin Leukemia, B-Cell Muromonab-CD3 Peripheral blood mononuclear cell (cell) Priming Exercise Protein Tyrosine Phosphatase Signal Transduction T-cell receptor complex location Tyrosine Phosphorylation |
| Content Type | Text |
| Resource Type | Article |
