NDLI: Tobacco (Nicotiana benthamiana Domin) Plant Transformation With and Without Selectable Marker Gene for Multiple Virus Resistance

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Tobacco (Nicotiana benthamiana Domin) Plant Transformation With and Without Selectable Marker Gene for Multiple Virus Resistance

Content Provider	Semantic Scholar
Author	Getu, Kassa
Copyright Year	2013
Abstract	Transformation of tobacco plants (Nicotiana benthamiana Domin), containing nontranslatable coat protein (CP) gene fragments of Potato virus Y (PVY), Potato leafroll virus (PLRV) and Potato virus X (PVX) with and without selectable marker gene was conducted using Agrobacteriummediated transformation. Preliminary identifications of the transformants were done based on the expression of the reporter gene, Beta-Glucuronidase (GUS) gene. GUS activity test was conducted in the first (T0) generation while the plants were in test tubes and after transferring to a greenhouse for marker-free and marker-aided transformations, respectively. In the transformation with marker gene, three lines, which later on (in T1 generation) became virus resistant and PCR-positive for the insert, were generated from GUS-negative mother plants. Although regeneration of plants from marker-free transformation was relatively easy, it was not possible to get transgenic plants using histochemical GUS staining selection system. Key words/phrases: Beta-Glucuronidase gene, potato leafroll virus, potato virus X, potato virus Y, tobacco (Nicotiana benthamiana)
Starting Page	17
Ending Page	26
Page Count	10
File Format	PDF HTM / HTML
Volume Number	36
Alternate Webpage(s)	https://www.ajol.info/index.php/sinet/article/download/89741/79199
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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