NDLI: Tobacco (Nicotiana benthamiana Domin) Plant Transformation With and Without Selectable Marker Gene for Multiple Virus Resistance

Content Provider	African Journals Online (AJOL)
Author	Kassa Getu
Abstract	Transformation of tobacco plants (Nicotiana benthamiana Domin), containing non-translatable coat protein (cp) gene fragments of Potato virus y (pvy), Potato leafroll virus (plrv) and Potato virus X (pvx) with and without selectable marker gene was conducted using Agrobacterium-mediated transformation. Preliminary identifications of the transformants were done based on the expression of the reporter gene, Beta-Glucuronidase (gus) gene. gus activity test was conducted in the first (T0) generation while the plants were in test tubes and after transferring to a greenhouse for marker-free and marker-aided transformations, respectively. In the transformation with marker gene, three lines, which later on (in T1 generation) became virus resistant and pcr-positive for the insert, were generated from gus-negative mother plants. Although regeneration of plants from marker-free transformation was relatively easy, it was not possible to get transgenic plants using histochemical gus staining selection system.
Ending Page	26
Starting Page	17
File Format	PDF
ISSN	03792897
e-ISSN	25207997
Journal	SINET: Ethiopian Journal of Science
Issue Number	1
Volume Number	36
Language	English
Publisher Date	2013-06-17
Access Restriction	Open
Subject Keyword	General Science Beta-Glucuronidase gene potato leafroll virus potato virus X potato virus Y tobacco (Nicotiana benthamiana)
Content Type	Text
Resource Type	Article
Subject	Decision Sciences

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Tobacco (Nicotiana benthamiana Domin) Plant Transformation With and Without Selectable Marker Gene for Multiple Virus Resistance