NDLI: Hydrophobic silicone elastomer chamber for recording trajectories of motile porcine sperms without adsorption.

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Hydrophobic silicone elastomer chamber for recording trajectories of motile porcine sperms without adsorption.

Content Provider	Semantic Scholar
Author	Matsuura, Koji Kuroda, Yuka Yamashita, Keisuke H. Funahashi, H.
Copyright Year	2011
Abstract	Motile porcine sperms adhere to hydrophilic materials such as glass and plastics. The adsorption of sperms to a hydrophobic poly(dimethylsiloxane) (PDMS) membrane is less compared with that to glass. We investigated the linear velocity (LV) and amplitude of lateral head displacement (ALHD) of motile porcine sperm on glass and PDMS preparations using computer-assisted sperm analysis (CASA). Significant decreases were observed in the 15-min LV (P<0.05) and ALHD (P<0.05) in motile porcine sperm on glass preparations compared with those on PDMS preparations. These differences were due to adsorption of the head and/or neck to hydrophilic substrates. Because of the elasticity of PDMS, we propose that a PDMS membrane should be used for CASA. To investigate the dynamics of motile porcine sperms with microfluidics, we do not recommend plasma treatment to bond PDMS and glass in the microchannel preparation; instead, we suggest that a PDMS molding process without plasma treatment be used for preparation of microfluidic channels.
Starting Page	1068
Ending Page	1073
Page Count	6
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.jstage.jst.go.jp/article/jrd/57/1/57_10-075N/_pdf
PubMed reference number	20953123v1
Volume Number	57
Issue Number	1
Journal	The Journal of reproduction and development
Language	English
Access Restriction	Open
Subject Keyword	Gastroenteritis, Transmissible, of Swine Linear IgA Bullous Dermatosis Microfluidics Plastics Porcine species Psychologic Displacement Silicone Elastomers Silicones Spermatozoa Cell Count Tissue membrane sperm cell
Content Type	Text
Resource Type	Article

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