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Secretion of follicle-stimulating hormone and production of inhibin are reciprocally related.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ying, Sin Y. Czvik, J. Becker, Annette Ling, Nicholas Ueno, Naoto Guillemin, Roger |
| Copyright Year | 1987 |
| Abstract | The production of inhibin in cultured granulosa cells from immature hypophysectomized, estrogen-treated rats and Sertoli cells from normal animals was determined by a specific radioimmunoassay using an antiserum against a synthetic replicate of [Tyr30]inhibin alpha-chain-(1-30). The amount of immunoreactive inhibin detected in the spent media of these cells is in proportion to the density of cells plated and the concentration of exogenously added follicle-stimulating hormone (FSH). In the presence of the estrogen precursor androstenedione (10(-7) M), FSH, but not luteinizing hormone, produced a dose-dependent increase in inhibin during 2-day culture of granulosa cells. In the absence of the estrogen precursor, similar but somewhat diminished inhibin production in responding to FSH was observed. Exogenously added estrogen potentiated the FSH-mediated release of inhibin in the absence of androstenedione. Neither androstenedione nor estradiol added to the cultured Sertoli cells had effect on inhibin production. A preparation of pure inhibin isolated on the basis of an in vitro bioassay and characterized chemically specifically suppressed serum FSH but not luteinizing hormone, when it was injected (24 micrograms per injection, two injections) into acutely ovariectomized rats. Thus, inhibin secreted by the granulosa and Sertoli cells specifically suppresses the secretion of pituitary FSH, and in turn FSH is primarily responsible for the inhibin production in these gonadal cells, as in a classical negative-feedback relationship. |
| Starting Page | 741 |
| Ending Page | 746 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/84/13/4631.full.pdf |
| PubMed reference number | 3110772v1 |
| Volume Number | 84 |
| Issue Number | 13 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adrenergic alpha-Agonists Androstenedione Biological Assay Cell secretion Estradiol Estrogens Follicle Stimulating Hormone Gonadal structure INHIBIN Immune Sera Ovarian Follicle Posterior Pituitary Hormones Stimulation (motivation) granulosa cell |
| Content Type | Text |
| Resource Type | Article |
