NDLI: MicroRNA-181a Inhibits Activated B-Cell-Like Diffuse Large B-Cell Lymphoma Progression by Repressing CARD11

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MicroRNA-181a Inhibits Activated B-Cell-Like Diffuse Large B-Cell Lymphoma Progression by Repressing CARD11

Content Provider	Semantic Scholar
Author	Zhu, Dan-Xia Fang, Changquan He, Wenting Wu, Chen Li, Xiaodong
Copyright Year	2019
Abstract	We investigated the role of miR-181a in diffuse large B-cell lymphoma (DLBCL) and its potential target genes. miR-181a levels were lower in activated B-cell- (ABC-) like DLBCL cells than that in germinal center B-cell- (GCB-) like DLBCL cells. Overexpression of miR-181a in ABC-like DLBCL cell lines (OCI-LY10 and ⤲) resulted in G0/G1 cell cycle arrest, increased apoptosis, and decreased invasiveness. miRNA target prediction programs (miRanda, TargetScan, and miRDB) identified caspase recruitment domain-containing protein 11 (CARD11) as a putative miR-181a target. CARD11 mRNA and protein levels were higher in the ABC-like DLBCL than that in GCB-like DLBCL. Moreover, CARD11 mRNA and protein levels were downregulated in the OCI-LY10 and ⤲ cell lines overexpressing miR-181a. Dual luciferase reporter assays confirmed the miR-181a binding site in the CARD11 3'UTR region. OCI-LY10 and ⤲ cells transfected with a CARD11 expression vector encoding miR-181a with a mutated binding site showed higher CARD11 protein levels, cell viability, G2/M phase cells, and invasiveness compared to those transfected with a wild-type CARD11 expression vector. Nude mice xenografted with OCI-LY10 cells with overexpressed wild-type miR-181a generated smaller tumors compared to those with overexpressed mutated binding site of CARD11 3'UTR and miR-181a. These results indicate that miR-181a inhibits ABC-like DLBCL by repressing CARD11.
File Format	PDF HTM / HTML
DOI	10.1155/2019/9832956
PubMed reference number	31662757
Journal	Medline
Volume Number	2019
Alternate Webpage(s)	http://downloads.hindawi.com/journals/jo/2019/9832956.pdf
Alternate Webpage(s)	https://doi.org/10.1155/2019%2F9832956
Journal	Journal of oncology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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