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A Reversed-phase High Performance Liquid Chromatographic Method for Determination of Chlorthalidone in Pharmaceutical Formulation
| Content Provider | Semantic Scholar |
|---|---|
| Author | Singh, Brijesh |
| Copyright Year | 2009 |
| Abstract | A simple, specific, sensitive, precise, and accurate high performance liquid chromatography method was developed for the determination of chlorthalidone in pharmaceutical tablet forms. Chlorthalidone is a diuretic drug frequently prescribed by Indian physicians for treatment of cardiac, hepatic, renal and pulmonary diseases. The method was carried out on reverse phase C-18 column (lichrospher, Merck ® ) (250×4mm, 5µm particle size) using a mixture of 50mM disodium hydrogen phosphate: methanol: acetonitrile in the ratio of 70:30:05 (pH adjusted to 3.5 with orthophosphoric acid) as mobile phase. Hydrochlorothiazide was used as internal standard. The detection was carried out by UV-detector at 220 nm at column temperature 30±2 0 C. The calibration curve was found to be linear in the range of 0.1 to 3.2 µg/ml. The intra-day and inter day percentage coefficient of variation was found 3.3085 and 0.3702 respectively. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ijppsjournal.com/Vol1Issue2/145.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
