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Microcoding: the second step in DNA barcoding.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Summerbell, Richard C. Lévesque, C. André Seifert, Keith A. Bovers, Marjan Fell, Jack W. Diaz, Mara R. Boekhout, Teun Hoog, Gert Sybren De Stalpers, Joost A. Crous, Pedro W. |
| Copyright Year | 2005 |
| Abstract | After the process of DNA barcoding has become well advanced in a group of organisms, as it has in the economically important fungi, the question then arises as to whether shorter and literally more barcode-like DNA segments should be utilized to facilitate rapid identification and, where applicable, detection. Through appropriate software analysis of typical full-length barcodes (generally over 500 base pairs long), uniquely distinctive oligonucleotide 'microcodes' of less than 25 bp can be found that allow rapid identification of circa 100-200 species on various array-like platforms. Microarrays can in principle fulfill the function of microcode-based species identification but, because of their high cost and low level of reusability, they tend to be less cost-effective. Two alternative platforms in current use in fungal identification are reusable nylon-based macroarrays and the Luminex system of specific, colour-coded DNA detection beads analysed by means of a flow cytometer. When the most efficient means of rapid barcode-based species identification is sought, a choice can be made either for one of these methodologies or for basic high-throughput sequencing, depending on the strategic outlook of the investigator and on current costs. Arrays and functionally similar platforms may have a particular advantage when a biologically complex material such as soil or a human respiratory secretion sample is analysed to give a census of relevant species present. |
| Starting Page | 461 |
| Ending Page | 464 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://pure.knaw.nl/portal/files/489995/20849.pdf |
| Alternate Webpage(s) | http://api.ning.com/files/vqfTY5ZlyIEexiJ5Cbl0l6uU9JGgr3aGEwuwhhdKdfH96zCDkzbrk3c6YJ-tbF25XUafe35Y5tb25dAu1lRM67Ne0olJQT9i/Phil.Trans.R.Soc.B2005Summerbell1897903.pdf |
| Alternate Webpage(s) | http://www.westerdijkinstitute.nl/images/ResearchGroups/Publications/2005Summerbell0001.pdf |
| PubMed reference number | 16214747v1 |
| Volume Number | 360 |
| Issue Number | 1462 |
| Journal | Philosophical transactions of the Royal Society of London. Series B, Biological sciences |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Bar Codes Base Pairing Bead Dosage Form Biopolymer Sequencing Censuses Fungi Nylons automated flow cytometers |
| Content Type | Text |
| Resource Type | Article |
