Controlling DNA capture and propagation through artificial nanopores.

Content Provider	Semantic Scholar
Author	Trepagnier, Eliane H. Radenovic, Aleksandra Sivak, David A. Geissler, Phillip L. Liphardt, Jan
Copyright Year	2007
Abstract	Electrophorescing biopolymers across nanopores modulates the ionic current through the pore, revealing the polymer's diameter, length, and conformation. The rapidity of polymer translocation ( approximately 30,000 bp/ms) in this geometry greatly limits the information that can be obtained for each base. Here we show that the translocation speed of lambda-DNA through artificial nanopores can be reduced using optical tweezers. DNAs coupled to optically trapped beads were presented to nanopores. DNAs initially placed up to several micrometers from the pore could be captured. Subsequently, the optical tweezers reduced translocation speeds to 150 bp/ms, about 200-fold slower than free DNA. Moreover, the optical tweezers allowed us to "floss" single polymers back and forth through the pore. The combination of controlled sample presentation, greatly slowed translocation speeds, and repeated electrophoresis of single DNAs removes several barriers to using artificial nanopores for sequencing, haplotyping, and characterization of protein-DNA interactions.
Starting Page	179
Ending Page	182
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.cchem.berkeley.edu/plggrp/papers/PLG2007b.pdf
Alternate Webpage(s)	https://infoscience.epfl.ch/record/129295/files/NanoLetters_Trepagnier_2007.pdf
PubMed reference number	17705552v1
Volume Number	7
Issue Number	9
Journal	Nano letters
Language	English
Access Restriction	Open
Subject Keyword	Bead Dosage Form Biopolymer Sequencing Biopolymers Diameter (qualifier value) Electrophoresis Optical Trap Polymers
Content Type	Text
Resource Type	Article