NDLI: Analysis of promoter activity of cotton lipid transfer protein gene LTP 6 in transgenic tobacco plants

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Analysis of promoter activity of cotton lipid transfer protein gene LTP 6 in transgenic tobacco plants

Content Provider	Semantic Scholar
Author	Hsu, Chris Ma, Din-Pow
Copyright Year	1999
Abstract	A cotton (Gossypium hirsutum) genomic clone (1.7-kb DNA insert) harboring the lipid transfer gene Ltp6 specifically expressed in fiber cells had been previously isolated and characterized. By using PCR amplification, the 447 bp Ltp6 promoter and a series of 5% deletions of the promoter were generated and cloned into a pBI101 plasmid upstream of the GUS (b-glucuronidase) reporter gene. These constructs were introduced into Agrobacterium tumefaciens LBA4404, and leaf disks of tobacco (Nicotiana tabacum L.) were transformed with A. tumefaciens LBA4404 carrying various promoter-GUS pBI101 plasmids. Histochemical analyses of the transgenic tobacco seedlings indicated that the Ltp6 promoter (from nt −447 to −1, undeleted) directed GUS expression only in trichomes (hair cells). Fluorometric GUS assays showed that the promoter activity of the undeleted Ltp6 promoter was at least 1000 times weaker than that of the 35S promoter of cauliflower mosaic virus (CaMV). Sequential deletions of the promoter gradually decreased the expression level of the GUS gene. No GUS activity was observed when the 5% deletion of the Ltp6 promoter reached to nt −86, which removed the putative CAAT and TATA promoter elements. © 1999 Elsevier Science Ireland Ltd. All rights reserved.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://naldc.nal.usda.gov/download/2833/PDF
Language	English
Access Restriction	Open
Subject Keyword	Agrobacterium tumefaciens Cauliflower Mosaic Virus Clone Cells Computer-aided audit tools Deletion Mutation Gene regulatory network Gossypium hirsutum Linux Test Project (LTP) Long-Term Potentiation Mosaic Organism Mosaic Viruses Nicotiana tabacum One Thousand Plasmids Seedlings Tissue fiber Trichomes
Content Type	Text
Resource Type	Article

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