NDLI: Effects of Mutagenesis in vitro on the Ability of Cloned Tomato Golden Mosaic Virus DNA to Infect Nicotiana benthamiana Plants

Please wait, while we are loading the content...

Effects of Mutagenesis in vitro on the Ability of Cloned Tomato Golden Mosaic Virus DNA to Infect Nicotiana benthamiana Plants

Content Provider	Semantic Scholar
Author	Brough, Clare L.
Copyright Year	1988
Abstract	Insertion mutations introduced in vitro into cloned DNA of tomato golden mosaic virus that considerably shortened the length of the open reading frames (ORFs) ALl, AL2/AL3, BL1 or BRI, abolished the ability of the DNA to infect Nicotiana benthamiana seedlings. Mutants in which ORF AR1 was similarly shortened by an insertion or a 28 bp deletion were infectious, showing that the formation of coat protein or virions is not required for replication and systemic spread of virus DNA, although the appearance of symptoms was delayed in infections with the deletion mutant. Mutants with larger deletions (178 bp to 603 bp) in ORF AR1 were not infectious. Infections could be initiated with mixtures of ALl and AL2/AL3 mutants, or BL1 and BRI mutants, primarily as a result of complementation, although a low proportion of wild-type DNA A molecules regenerated by recombination or reversion was detected in the progeny of infection with the DNA A mutants.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://vir.sgmjournals.org/content/69/3/503.full.pdf
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/jgv/69/3/JV0690030503.pdf?accname=guest&checksum=E89FED554F87993DD7C8B99DC26A9EA8&expires=1544490621&id=id
Language	English
Access Restriction	Open
Subject Keyword	Clone Cells Crossover (genetic algorithm) DNA Fingerprinting Deletion Mutation Ecthyma, Contagious Frame (physical object) Infection Insertion Mutation Large Mosaic - computer software Mosaic Organism Mosaic Viruses Murine sarcoma viruses Nicotiana tabacum Ab.IgE:ACnc:Pt:Ser:Qn Open Reading Frames Open reading frame Progeny Linux Systems Reading Frames (Nucleotide Sequence) Reversion (software development) Seedlings Tomato golden mosaic virus Virion mixture mutant
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in