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Análisis metagenómico de la evolución de las comunidades microbianas en alimentos sometidos a refrigeración y en condiciones de ausencia de frío
| Content Provider | Semantic Scholar |
|---|---|
| Author | Pires, Virgínia M. R. Rivas, Raúl García-Fraile, Paula |
| Copyright Year | 2019 |
| Abstract | The cold chain is a key element in food safety since most microorganisms stop their growth at low temperatures. Techniques that analyse the bacterial populations of a sample by sequencing a fragment of the 16S ribosomal gene, allowing the microbial characterization of foods have been developed. The objective of the present study was to identify and quantify bacteria on refrigerated and uncooled foods using massive sequencing of rDNA16S, to describe the role of maintenance of the cold chain on the evolution of microbial communities in food. Thus, the DNA extracted from the samples was sequenced at 2d and 4d. The OTUs obtained were compared with those of the databases and identified. The culture of microorganisms of the samples was carried out at 0d. In general, an increase in the amount and diversity of bacteria was observed in the uncooled samples, confirming that the cold chain delays the development of microorganisms in foods, increasing the shelf life of them. In addition, the study demonstrates the efficacy of the massive sequencing of rDNA16S to study the effect of temperature on the development of bacterial communities on food. |
| Starting Page | 73 |
| Ending Page | 84 |
| Page Count | 12 |
| File Format | PDF HTM / HTML |
| DOI | 10.14201/fj2019427384 |
| Volume Number | 4 |
| Alternate Webpage(s) | http://revistas.usal.es/index.php/2445-1355/article/download/fj2019427384/20505 |
| Alternate Webpage(s) | https://doi.org/10.14201/fj2019427384 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
