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Selección de los marcadores cultivables y moleculares útiles para diferenciar el origen de la contaminación fecal en el Río Bogotá
| Content Provider | Semantic Scholar |
|---|---|
| Author | Alfonso, Sánchez Catherine, Andrea |
| Copyright Year | 2017 |
| Abstract | The detection of intestinal pathogenic microorganisms in water is difficult due to the high cost of testing and the time of analysis. As an alternative, indicator microorganisms are used, which do not differentiate if the contamination is of human or animal fecal origin. To discriminate the origin of fecal contamination, it has been proposed the use of culture-dependent and molecular markers that may present geographic specificity, so it is necessary to identify those applicable in our environment. The objective of this work was to select the cultivable and molecular markers to differentiate the origin of the fecal contamination in the Bogota river. For this a strain was isolated from porcine intestinal contents identified as Bacteroides fragilis PZ8. Non-discriminatory indicators as: total coliforms, E. coli, somatic coliphages and Bacteroides fragilis phages strain RYC2056, discriminant indicators: Bacteroides thetaiotaomicron strain GA17 and Bacteroides fragilis PZ8 phages, and molecular markers: HF183F, CF128F and Bifidobacterium adolescentis (ADO) and Bifidobacterium dentium (DEN), by PCR, in wastewater from bovine and porcine abattoir, wastewater treatment plants (WWTP) and Bogota river. It was evidenced that strains GA17 and PZ8 have the potential to discriminate human and porcine fecal contamination, respectively, in the evaluated waters. The relationship between somatic coliphages and phage strain GA17 of two units, showed that the contamination of the Bogota river is mainly of human origin. The molecular markers HF183F, ADO and DEN indicated the presence of human contamination and the marker CF128F, the presence of bovine contamination in the evaluated waters. The selected markers are potentially useful to discriminate the origin of fecal contamination in the Bogota river. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://repository.javeriana.edu.co/bitstream/handle/10554/38611/SanchezAlfonsoAndreaCatherine2017.pdf?isAllowed=y&sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
