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A role for initiation codon context in chloroplast translation.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Esposito, Donna Hicks, Amanda J. Stern, David B. |
| Copyright Year | 2001 |
| Abstract | To study the role of initiation codon context in chloroplast protein synthesis, we mutated the three nucleotides immediately upstream of the initiation codon (the -1 triplet) of two chloroplast genes in the alga Chlamydomonas reinhardtii. In prokaryotes, the -1 triplet has been proposed to base pair with either the 530 loop of 16S rRNA or the extended anticodon of fMet-tRNA. We found that in vivo, none of the chloroplast mutations affected mRNA stability. However, certain mutations did cause a temperature-sensitive decrease in translation and a more dramatic decrease at room temperature when combined with an AUU initiation codon. These mutations disrupt the proposed extended base pairing interaction with the fMet-tRNA anticodon loop, suggesting that this interaction may be important in vivo. Mutations that would still permit base pairing with the 530 loop of the 16S rRNA also had a negative effect on translation, suggesting that this interaction does not occur in vivo. Extended base pairing surrounding the initiation codon may be part of a mechanism to compensate for the lack of a classic Shine-Dalgarno rRNA interaction in the translation of some chloroplast mRNAs. |
| Starting Page | 13 |
| Ending Page | 13 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.plantcell.org/content/plantcell/13/10/2373.full.pdf |
| PubMed reference number | 11595808v1 |
| Volume Number | 13 |
| Issue Number | 10 |
| Journal | The Plant cell |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Algae Anticodon Base Pairing Chloroplasts Codon (nucleotide sequence) Codon, Initiator Cognitive function: initiation Genes, Chloroplast Genetic Translation Process Lobochlamys segnis Mutation Nucleotides Prokaryote Protein Biosynthesis Transcription Initiation |
| Content Type | Text |
| Resource Type | Article |
