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A poly(A) binding protein functions in the chloroplast as a message-specific translation factor.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Cohen, Amybeth Danon, Avihai Mayfield, Stephen Patrick |
| Copyright Year | 1998 |
| Abstract | High-affinity binding of a set of proteins with specificity for the 5' untranslated region (UTR) of the Chlamydomonas reinhardtii chloroplast psbA mRNA correlates with light-regulated translational activation of this message. We have isolated a cDNA encoding the main psbA RNA binding protein, RB47, and identified this protein as a member of the poly(A) binding protein family. Poly(A) binding proteins are a family of eukaryotic, cytoplasmic proteins thought to bind poly(A) tails of mRNAs and play a role in translational regulation. In vitro translation of RNA transcribed from the RB47 cDNA produces a precursor protein that is efficiently transported into the chloroplast and processed to the mature 47-kDa protein. RB47 expressed and purified from Escherichia coli binds to the psbA 5' UTR with similar specificity and affinity as RB47 isolated from C. reinhardtii chloroplasts. The identification of a normally cytoplasmic translation factor in the chloroplast suggests that the prokaryotic-like chloroplast translation machinery utilizes a eukaryotic-like initiation factor to regulate the translation of a key chloroplast mRNA. These data also suggest that poly(A) binding proteins may play a wider role in translation regulation than previously appreciated. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/95/5/2238.full.pdf |
| PubMed reference number | 9482869v1 |
| Volume Number | 95 |
| Issue Number | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 5' Untranslated Regions Affinity Chloroplasts DNA, Complementary F Factor Genetic Translation Process Lobochlamys segnis Peptide Initiation Factors Protein Family RNA Tail Transcription Initiation Translational Activation Translational Regulation cytoplasmic translation translation factor |
| Content Type | Text |
| Resource Type | Article |
