NDLI: Fluorescent Viability Assay of Mycobacterium avium subsp. paratuberculosis by carboxyfluorescein diacetate succinimidyl ester and carboxyfluorescein diacetate

Please wait, while we are loading the content...

Fluorescent Viability Assay of Mycobacterium avium subsp. paratuberculosis by carboxyfluorescein diacetate succinimidyl ester and carboxyfluorescein diacetate

Content Provider	Semantic Scholar
Author	Wang, Xoiafei Wang, Hongyu Momotani, Yuriko Momotani, Eiichi
Copyright Year	2009
Abstract	Mycobacterium avium subspecies paratuberculosis (Map) is an important pathogen causing ruminant paratuberculosis. Paratuberculosis is characterized by chronic granulomatous enteritis, persistent diarrhea, progressive wasting, and finally death, and as resulted in significant economic losses to the dairy and cattle industries worldwide. Furthermore, Map is speculated to be the cause of human Crohn’s disease. One of the difficulties of Map in diagnosis and research is its significant slow growth. Therefore, we investigated the application of a viability test of Map by Carboxyfluorescein diacetate succinimidyl (CFDA) and carboxyfluores cein diacetate succinimidyl ester (CFDA/ SE) labeling as a quick method. The results revealed that CFDA is a useful reagent as a fluorescent probe to determine the quan titative viability of Map but unsatisfactory results obtained with CFDA/SE. Incubation of Map with CFDA at 100μM for 30min was practically the optimal condition for the viability test. We suggest that this method will be used widely as a simplified semi-quanti tative viability assay in evaluation of effect of anti-bacterial substance, disinfectant or bactericidal activity of macrophages.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.jarvm.com/articles/Vol7Iss4/Vol7%20Iss4momotani.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in